Product Details

Purity

>85% (SDS-PAGE)

Target Names

Tigar

Uniprot No.

Q8BZA9

Alternative Names

Tigar; Fructose-2,6-bisphosphatase TIGAR; EC 3.1.3.46; TP53-induced glycolysis and apoptosis regulator; TP53-induced glycolysis regulatory phosphatase

Species

Mus musculus (Mouse)

Expression Region

1-269

Target Protein Sequence

MPRFALTVIR HGETRLNKEK IIQGQGVDAP LSETGFRQAA AAGQFLSNVQ FTHAFSSDLT RTKQTIHGIL EKSRFCKDMA VKYDSRLRER MYGVAEGKPL SELRAMAKAA GEECPMFTPP GGETVEQVKM RGKDFFDFIC QLILGKAGQR ESVLPGAPGS GLESSLAEVF PVGKHGSLGA NPKGGTLGLA ASILVVSHGA YMRSLFGYFL SDLRCSLPGA RDKLELSSIT PNTGISVFII DCEEARQPSI QCVCMNLQEH LNGVTEKQH

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate. Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production. Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death. Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity. In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage. Plays a role in adult intestinal regeneration; contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation. Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions. Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner. Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses. Involved in intestinal tumor progression.

Gene References into Functions

Study demonstrated that TIGAR played a positive role in survival of astrocytes and a negative role in inflammatory response after stroke attack. TIGAR exerted these functions through increasing flux of pentose phosphate pathway and inhibiting NF-kappaB activation. PMID: 29331305
TIGAR contributes to ischemic tolerance induced by cerebral preconditioning through scavenging of reactive oxygen species and inhibition of apoptosis. PMID: 27256465
Results suggest that TIGAR expression changes during development and its expression level may be correlated with the vulnerability of neurons to ischemic injury. PMID: 26219221
Although mouse TIGAR expression is clearly induced in the intestines of mice following DNA-damaging stress of ionizing radiation, that was not dependent on p53 or TAp73. PMID: 26247727
TIGAR protein expression in brain is increased following ischemia reperfusion injury. PMID: 25445985
Therefore, we conclude that TIGAR knockdown-induced radiosensitization of glioma cells may be dependent on the inhibition of TRX1 nuclear translocation. PMID: 24509157
TIGAR protects ischemic brain injury and preserves mitochodrial function. PMID: 24872551
TIGAR has roles in efficient intestinal regeneration and tumorigenesis PMID: 23726973
p53/TIGAR-mediated inhibition of myocyte mitophagy is responsible for impairment of mitochondrial integrity and subsequent apoptosis. PMID: 22044588
p53 and TIGAR inhibit glycolysis in hypoxic myocytes and that inhibition of glycolysis is closely involved in apoptosis, suggesting that p53 and TIGAR are significant mediators of cellular energy homeostasis and cell death under ischemic stress. PMID: 20935145

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Subcellular Location

Cytoplasm. Nucleus. Mitochondrion.

Protein Families

Phosphoglycerate mutase family

Tissue Specificity

Expressed in olfactory bulb, cerebellum, and cortex. Expressed in neurons and astrocytes (at protein level). Expressed in intestinal crypt.

Database Links

KEGG: mmu:319801

STRING: 10090.ENSMUSP00000048643

UniGene: Mm.101836

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Recombinant Mouse Probable fructose-2,6-bisphosphatase TIGAR (Tigar)

Product Details

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Target Background

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