Recombinant Mouse Protein bicaudal C homolog 1 (Bicc1), partial

Code CSB-YP858155MO
MSDS
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Source Yeast
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Code CSB-EP858155MO
MSDS
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Source E.coli
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Code CSB-EP858155MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP858155MO
MSDS
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Source Baculovirus
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Code CSB-MP858155MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Bicc1
Uniprot No.
Alternative Names
Bicc1Protein bicaudal C homolog 1; Bic-C
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Putative RNA-binding protein. May be involved in regulating gene expression during embryonic development.
Gene References into Functions
  1. Polymerization is a novel disease-relevant mechanism both to stabilize Bicc1 and to present associated mRNAs in specific silencing platforms. PMID: 26217012
  2. discovered that loss of the Pkd1 gene product, polycystin-1 (PC1), whose mutation causes human autosomal dominant polycystic kidney disease (ADPKD), downregulates Bicc1 expression in vitro and in vivo PMID: 24594709
  3. identify Bicc1 as a genetic determinant of osteoblastogenesis and BMD and suggest that it does so by regulating Pkd2 transcript levels PMID: 24789909
  4. Bicc1 is a novel regulator of osteoblastogenesis and bone mineral density (BMD) PMID: 24789909
  5. Bicc1 similarly induces silencing of the protein kinase inhibitor PKIalpha by miR-27a. PMID: 22641646
  6. Bicc1 KH domains bind the 3' UTRs of AC6 and PKIa mRNAs and cognate miRNA precursors, whereas the SAM domain loads a silencing complex with AGO2. AC6 and PKIa protein levels increase in cystic Bicc1-/- mouse kidneys. PMID: 22641646
  7. lack of Bicc1 leads to disruption of normal cell-cell junctions. PMID: 20219263
  8. Data suggest that Bicc1 functions by modulating the expression of polycystin 2 (Pkd2 by antagonizing the repressive activity of the miR-17 microRNA family on the 3'UTR of Pkd2 mRNA. PMID: 20215348
  9. Binds homoribopolymers in vitro; the third K homology domain is necessary and sufficient for in vitro Bicc1 RNA binding. PMID: 18182784
  10. Bicc1 and SamCystin interact at the protein level suggesting that they function in a common molecular pathway that when perturbed, is involved in cystogenesis. PMID: 19324013
  11. Data report that targeted inactivation of BicC randomizes left-right asymmetry by disrupting the planar alignment of motile cilia required for cilia-driven fluid flow, and that BicC can uncouple Dvl2 signaling from the canonical Wnt pathway. PMID: 19666828

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Subcellular Location
Cytoplasm.
Protein Families
BicC family
Tissue Specificity
In the adult, predominantly expressed in heart and kidney. In 8 week old mice, expressed in growing primary oocytes and in the stromal cells of the theca.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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