Recombinant Mouse Protein dpy-30 homolog (Dpy30)

Code CSB-YP857493MO
MSDS
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Source Yeast
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Code CSB-EP857493MO
MSDS
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Source E.coli
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Code CSB-EP857493MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP857493MO
MSDS
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Source Baculovirus
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Code CSB-MP857493MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dpy30
Uniprot No.
Alternative Names
Dpy30Protein dpy-30 homolog; Dpy-30-like protein; Dpy-30L
Species
Mus musculus (Mouse)
Expression Region
1-99
Target Protein Sequence
MESEQMLEGQ TQVAENPHSE YGLTDSVERI VENEKINAEK SSKQKVDLQS LPTRAYLDQT VVPILLQGLA VLAKERPPNP IEFLASYLLK NKAQFEDRN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
As part of the MLL1/MLL complex, involved in the methylation of histone H3 at 'Lys-4', particularly trimethylation. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. May play some role in histone H3 acetylation. In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and histone H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal. May also play an indirect or direct role in endosomal transport.
Gene References into Functions
  1. Dpy30-deficient hematopoietic stem and progenitor cells cannot differentiate or efficiently up-regulate lineage-regulatory genes, and eventually fail to sustain for long term. PMID: 27647347
  2. Dpy30 role in cellular reprogramming and its interactions with Set1 complex PMID: 26691508
  3. Results indicate an essential functional role for Dpy-30 and SET1/MLL complex-mediated H3K4 methylation, as a component of the bivalent mark, at developmental genes during the embryonic stem cells fate transitions. PMID: 21335234
Subcellular Location
Nucleus. Golgi apparatus, trans-Golgi network. Note=Associated with chromatin at regions enriched in histone H3 trimethylated at 'Lys-4'. Highly enriched in gene promoter regions and 5' UTRs, but not in downstream regions of genes or 3' UTRs.
Protein Families
Dpy-30 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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