Recombinant Mouse Protein regulator of cytokinesis 1 (Prc1)

Code CSB-YP018633MO
MSDS
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Source Yeast
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Code CSB-EP018633MO
MSDS
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Source E.coli
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Code CSB-EP018633MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018633MO
MSDS
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Source Baculovirus
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Code CSB-MP018633MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Prc1
Uniprot No.
Alternative Names
Prc1; Protein regulator of cytokinesis 1
Species
Mus musculus (Mouse)
Expression Region
1-603
Target Protein Sequence
MRRSEVLADE SITCLQKALT HLREIWELIG IPEEQRLQRT EVVKKHIKDL LDRMIAEEES LRERLLKSIS ICQKELSTLC SELQVKPFQE EKDTTILQLE KDLRTQVELM RKQKKERKQE LKLLQEQEQE LRDILCMPPC DVDSTSVPTL EELKLFRQRV ATLRETKESR REEFVNIKKQ IILCMEELEH SPDTSFERDV VCEDESAFCL SLENIATLQK LLKQLEMKKS QNEAECEGLR TQIRELWDRL QIPEEEREPV EAIMTGSKTK IRNALKLEVD RLEELKMQNI KQVIEKIRVE LAQFWDQCFY SQEQRQAFAP YYSEDYTENL LHLHDAEIVR LRNYYDVHKE LFQGVQKWEE SWKLFLEFER KASDPGRFTN RGGNLLKEEK ERAKLQKTLP KLEEELKARI EQWEQEHSTA FVVNGQKFME YVTEQWELHR LEKERAKQER QLKNKKQTEA EMLYGSTPRT PSKRPGQTPK KSGKMNTTTM SSATPNSSIR PVFGGSVYRS PMSRLPPSGS KSVVTSLCSG KKTPRAAQLR ANKENLDLNG SILSGGYPGS TPLQHNCSIK SVASTYSEFS RELSKASRSD ATSRILNSTN IQS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.
Gene References into Functions
  1. Authors analyzed whether elevated levels of the polycomb repressor complex 1 (PRC1) components Bmi1 and Ring1b and their catalyzed histone modification H2AK119ub in ADMs and tumor cells, are responsible for the mediation of acinar gene silencing. PMID: 26716510
  2. Here, the authors discover that the histone H2AK119 E3 ubiquitin ligase activity of Polycomb repressive complex 1 (PRC1) is defined by the composition of its catalytic subunits and is highly regulated by RYBP/YAF2-dependent stimulation. PMID: 27705745
  3. Here, by combining live-cell single-molecule tracking (SMT) and genetic engineering, the authors reveal that H3K27me3 contributes significantly to the targeting of Cbx7-PRC1 complex to chromatin. PMID: 27723458
  4. The data reveal how the interplay between PRC1, ncRNA and Mediator complexes controls pluripotency and cellular differentiation. PMID: 27096886
  5. our findings show that PRC1 mediates gene repression by combining multiple and different effector mechanisms, of which H2A ubiquitination is a major contributor PMID: 22844243

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Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Midbody.
Protein Families
MAP65/ASE1 family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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