Recombinant Mouse Purkinje cell protein 2 (Pcp2)

Code CSB-YP017635MO
MSDS
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Source Yeast
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Code CSB-EP017635MO
MSDS
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Source E.coli
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Code CSB-EP017635MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017635MO
MSDS
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Source Baculovirus
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Code CSB-MP017635MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pcp2
Uniprot No.
Alternative Names
Pcp2; Pcp-2; Purkinje cell protein 2; Protein PCD-5; Purkinje cell-specific protein L7
Species
Mus musculus (Mouse)
Expression Region
1-120
Target Protein Sequence
MAGSPDQEGF FNLLTHVQGD RMEEQRCSLQ AGPGQNPESQ GGPAPEMDNL MDMLVNTQGR RMDDQRVTVN SLPGFQPIGP KDGMQKRPGT LSPQPLLTPQ DPAALSFRRN SSPQPQTQAP
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May function as a cell-type specific modulator for G protein-mediated cell signaling.
Gene References into Functions
  1. A novel Pcp2 transcript is particular to mouse cerebellar Purkinje cells and retinal bipolar neurons. PMID: 27525924
  2. Study describes alternative splicing of the pcp2/L7 gene; biochemical properties of short and long form differ PMID: 24092530
  3. The results of this study showed that L7-PP2B mice, which suffer from impaired intrinsic plasticity and synaptic potentiation of their Purkinje cells. PMID: 24478374
  4. This study characterized the Cre-positive retinal ganglion cells in a PCP2 (Purkinje cell protein 2)-cre mouse line. We found that all of the Cre-positive retinal ganglion cells were bistratified retinal ganglion cells. PMID: 23224947
  5. both L7 genotype and sex contribute to both acquisition and extinction of fear conditioning is affected in Pcp2(L7) mutants PMID: 22528962
  6. Two mRNA forms predict two forms of the L7/Pcp-2 protein, which are both highly conserved across species.In mouse both mRNA forms are detectable in dendrites but their relative proportions change during development. PMID: 12399102
  7. colocalization and association of Galpha(o) and Pcp2 in cerebellum suggest a functional role in regions of synaptic activity. PMID: 12424730
  8. BAC/Cre transgenic mice have exclusive Cre expression in Purkinje and bipolar cells PMID: 15354293
  9. effect on expression of cre recombinase PMID: 15883005
  10. Pcp2 induces differentiation in PC12 cells, in part through Gbetagamma-mediated Ras and p38 MAPK activation and suggest the potential for similar signalling mechanisms in Purkinje cells. PMID: 15948714
  11. We conclude that PCP2 facilitates cation channels closure in the dark, shortens the rise time of the light response directly, and accelerates the decay time indirectly via the inhibitory network. PMID: 18768681
  12. Results suggest that the Pcp2(L7) protein acts as a sensorimotor damper that modulates time- and sense-dependent changes in motor responses. PMID: 18930827

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Tissue Specificity
Cerebellum (Purkinje cells) and retinal bipolar neurons.
Database Links
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