Recombinant Mouse Putative methyltransferase NSUN5 (Nsun5)

Code CSB-YP810399MO
MSDS
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Source Yeast
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Code CSB-EP810399MO
MSDS
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Source E.coli
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Code CSB-EP810399MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP810399MO
MSDS
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Source Baculovirus
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Code CSB-MP810399MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nsun5
Uniprot No.
Alternative Names
Nsun5; Wbscr20; Wbscr20a; Probable 28S rRNA; cytosine-C(5))-methyltransferase; EC 2.1.1.-; NOL1/NOP2/Sun domain family member 5; Williams-Beuren syndrome chromosomal region 20A protein homolog
Species
Mus musculus (Mouse)
Expression Region
2-465
Target Protein Sequence
GLYAAAAAV LAGVESRQGS LKGLVYSSNF QNLKQLYALV CETQRYSAVL DAVIASAGLL RAEKKLRPHL AKVLVYELLL GKGFRGGGGR WKALLGRHQA RLKAELARLK VHRGVSRNED LLQESSRPGQ AYQVPRFVRV NTLKTRPEDA IDYFKRQGFS YQGRASSLED LRALKGQHFL LDPLLPELLV FPAQTDLHEH PLYRAGHLIL QDKASCLPAM LLSPPPGSHV IDACAAPGNK TSYIAALLKN QGKIFAFDQD AKRLAAMATL VARAGVSCCE LAEKDFLTVS PSDQRYSQVQ YILLDPSCSG SGMLSRQLEE HGEGTPSKER LQALAGFQQR ALCHALRFPS LQRLVYSTCS LCQEENEDVV QEALQHNSGT FRLAPVLPTW PHRGLSTFPG SEHCLRASPE TTLTGGFFIA VFERAEVVPT PAPQTDAMDP EPLSQVPKRK RRRKAAVGAS MQPST
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 3438 (m5C3438) in 28S rRNA. m5C3782 promotes protein translation without affecting ribosome biogenesis and fidelity. Required for corpus callosum and cerebral cortex development.
Gene References into Functions
  1. mouse homologues of two novel genes, WBSCR20 and WBSCR22, deleted in Williams-Beuren syndrome (WBSCR20; WBSCR22) PMID: 11978965
Subcellular Location
Nucleus, nucleolus.
Protein Families
Class I-like SAM-binding methyltransferase superfamily, RsmB/NOP family
Tissue Specificity
In the hippocampus, specifically expressed in adult hippocampal NG2-positive oligodendrocyte precursor cells (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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