Product Details

Purity

>85% (SDS-PAGE)

Target Names

Rabgef1

Uniprot No.

Q9JM13

Alternative Names

Rabgef1; Rabex5; Rab5 GDP/GTP exchange factor; Rabex-5

Species

Mus musculus (Mouse)

Expression Region

1-491

Target Protein Sequence

MSLKSERRGI HVDQSELLCK KGCGYYGNPA WQGFCSKCWR EEYHKARQRQ IQEDWELAER LQREEEEAFA SSQSSQGAQS LTFSKFEEKK TNEKTRKVTT VKKFFSASSR AGSKKEIQEA KAPSPSINRQ TSIETDRVTK EFIDFLKTFH KTGQEVYKQT KMFLEAMPYK RDLSIEEQSE CTQDFYQNVA ERMQTRGKVP PEKVEKIMDQ IEKHIMTRLY KFVFCPETTD DEKKDLAIQK RIRALHWVTP QMLCVPVNEE IPEVSDMVVK AITDIIEMDS KRVPRDKLAC ITRCSKHIFN AIKITKNEPA SADDFLPTLI YIVLKGNPPR LQSNIQYITR FCNPSRLMTG EDGYYFTNLC CAVAFIEKLD AQSLNLSQED FDRYMSGQTS PRKQESESWP PEACLGVKQM YKNLDLLSQL NERQERIMNE AKKLEKDLID WTDGIAKEVQ DIVEKYPLEI KPPNQPLAAI DSENVENDKL PPPLQPQVYA G

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase.

Gene References into Functions

AP-1/sigma1A-ArfGAP1-Rabex-5 complex formation leads to more endosomal Rabex-5 and enhanced, Rab5(GTP)-stimulated Vps34 PI3-kinase activity, which is essential for multivesicular body endosome formation. PMID: 27411398
findings reveal a key role for RABGEF1 in dampening keratinocyte-intrinsic MYD88 signaling and sustaining epidermal barrier function PMID: 27820702
Knockdown of RABEX-5 also inhibited wound healing, migration and the invasive abilities of gastric cancer cells. PMID: 25427001
Kruppel-like factor 5 is indispensable for adhesion, homing, lodging and retention of haematopoietic stem cells and progenitors in the bone marrow through Rab5-dependent post-translational regulation of beta1/beta2 integrins PMID: 23552075
Rab5 is required for phagocytosis of heat-inactivated Pseudomonas aeruginosa by macrophages. PMID: 23630954
a novel mechanistic insight into how Rabex-5 regulates internalization and postendocytic trafficking of ubiquitinated L1 destined for lysosomal degradation. PMID: 22846990
Data report the identification of Rab22 as a binding site on early endosomes for direct recruitment of Rabex-5 and activation of Rab5, establishing a Rab22-Rab5 signaling relay to promote early endosome fusion. PMID: 19759177
RabGEF1 is a negative regulator of Fc epsilon RI-dependent mast cell activation, and a lack of RabGEF1 results in the development of skin inflammation in vivo. PMID: 15235600
p85alpha protein may play a role in the down-regulation of activated receptors through its temporal control of the GTPase cycles of Rab5 and Rab4 PMID: 15377662
RabGEF1 plays a critical role in the regulation of SCF/c-Kit-mediated signaling events and biological responses in mast cells. PMID: 16533754
Data show that RabGEF1 is a negative regulator of Ras signalling and FcepsilonRI-dependent mast cell activation in vitro, and a lack of RabGEF1 results in the development of elevated numbers of mast cells in the skin and severe skin inflammation in vivo. PMID: 16605131
Insulin-stimulated Interaction between insulin receptor substrate 1 and p85alpha and activation of protein kinase B/Akt require Rab5. PMID: 16880210
Our data demonstrate that TeNT H(C) uses a retrograde transport pathway shared with p75(NTR), TrkB, and BDNF, which is strictly dependent on the activities of both Rab5 and Rab7 PMID: 17046692

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Subcellular Location

Cytoplasm. Early endosome. Recycling endosome.

Tissue Specificity

Expressed in the white matter tracts of the cerebellum, the fimbria hippocampi and the corpus callosum.

Database Links

KEGG: mmu:56715

STRING: 10090.ENSMUSP00000026390

UniGene: Mm.288639

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Recombinant Mouse Rab5 GDP/GTP exchange factor (Rabgef1)

Product Details

Customer Reviews and Q&A

Target Background

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