Product Details

Purity

>85% (SDS-PAGE)

Target Names

Rsad2

Uniprot No.

Q8CBB9

Alternative Names

Rsad2; Vig1; Radical S-adenosyl methionine domain-containing protein 2; Viperin; Virus inhibitory protein; endoplasmic reticulum-associated; interferon-inducible

Species

Mus musculus (Mouse)

Expression Region

1-362

Target Protein Sequence

MGMLVPTALA ARLLSLFQQQ LGSLWSGLAI LFCWLRIALG WLDPGKEQPQ VRGEPEDTQE TQEDGNSTQP TTPVSVNYHF TRQCNYKCGF CFHTAKTSFV LPLEEAKRGL LLLKQAGLEK INFSGGEPFL QDRGEYLGKL VRFCKEELAL PSVSIVSNGS LIRERWFKDY GEYLDILAIS CDSFDEQVNA LIGRGQGKKN HVENLQKLRR WCRDYKVAFK INSVINRFNV DEDMNEHIKA LSPVRWKVFQ CLLIEGENSG EDALREAERF LISNEEFETF LERHKEVSCL VPESNQKMKD SYLILDEYMR FLNCTGGRKD PSKSILDVGV EEAIKFSGFD EKMFLKRGGK YVWSKADLKL DW

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Interferon-inducible antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Catalyzes the conversion of cytidine triphosphate (CTP) to 3'-deoxy-3',4'-didehydro-CTP (ddhCTP) via a SAM-dependent radical mechanism. In turn, ddhCTP acts as a chain terminator for the RNA-dependent RNA polymerases from multiple viruses and directly inhibits viral replication. Therefore, inhibits a wide range of DNA and RNA viruses. Promotes also TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating 'Lys-63'-linked ubiquitination of IRAK1 by TRAF6. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins.

Gene References into Functions

The viperin putative active site contains several conserved positively charged residues, and a portion of the active site shows structural similarity to the GTP-binding site of MoaA, suggesting that the viperin substrate may be a nucleoside triphosphate of some type. PMID: 28607080
miR-200 family may potentially promote podocyte differentiation through repression of RSAD2 expression. PMID: 27251424
A relatively high level of viperin protein expression was detected in infected RAW 264.7 cells, and it was extensively localized throughout the cytoplasm of infected cells PMID: 25433308
The data indicate that viperin is the major effector underlying the ability of HCMV to regulate cellular lipid metabolism. PMID: 23935494
The viperin-deficient mouse model indicated that absence of viperin enhanced neither the viral load nor pulmonary damage in the lungs. PMID: 22377585
Viperin(-/-) adult mice infected with West Nile virus via the subcutaneous or intracranial route showed increased lethality and/or enhanced viral replication in central nervous system (CNS) tissues. PMID: 21880757
study shows human cytomegalovirus (HCMV)-induced viperin disrupts cellular metabolism to enhance infectious process; viperin interaction with vMIA resulted in viperin relocalization from endoplasmic reticulum to mitochondria PMID: 21527675
Viperin may play a central role in bacterial or parasitic infections and may protect neutrophils and macrophages from infection. PMID: 20410488
Vesicular stomatitis virus (VSV)-mediated viperin induction occurs independently of interferon (IFN), through IFN regulatory factor (IRF)-1; the transcriptional upregulation of viperin is sufficient to reduce VSV replication. PMID: 20308629
Viperin is expressed in atherosclerosis and induced in vascular cells by inflammatory stimuli and cytomegalovirus infection PMID: 15890971
viperin facilitates TCR-mediated GATA-3 activation and optimal Th2 cytokine production by modulating NF-kappaB and AP-1 activities PMID: 19047684

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Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus. Endoplasmic reticulum. Lipid droplet. Mitochondrion. Mitochondrion inner membrane. Mitochondrion outer membrane.

Protein Families

Radical SAM superfamily, RSAD2 family

Tissue Specificity

Expressed at higher levels in atherosclerotic arteries than in normal arteries.

Database Links

KEGG: mmu:58185

STRING: 10090.ENSMUSP00000020970

UniGene: Mm.24045

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Recombinant Mouse Radical S-adenosyl methionine domain-containing protein 2 (Rsad2)

Product Details

Customer Reviews and Q&A

Target Background

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