Recombinant Mouse Rho GTPase-activating protein 33 (Arhgap33), partial

Code CSB-YP800366MO
MSDS
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Source Yeast
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Code CSB-EP800366MO
MSDS
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Source E.coli
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Code CSB-EP800366MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP800366MO
MSDS
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Source Baculovirus
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Code CSB-MP800366MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Arhgap33
Uniprot No.
Alternative Names
Arhgap33; Snx26; Tcgap; Rho GTPase-activating protein 33; Rho-type GTPase-activating protein 33; Sorting nexin-26; Tc10/CDC42 GTPase-activating protein
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May be involved in several stages of intracellular trafficking. Could play an important role in the regulation of glucose transport by insulin. May act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.
Gene References into Functions
  1. ARHGAP33 interacts with SORT1 to cooperatively regulate TrkB trafficking. ARHGAP33 KO mice exhibit reduced expression of synaptic TrkB, impaired dendritic spine development and neuropsychiatric disorder-related behavioural abnormalities. PMID: 26839058
  2. Results indicate that SNX26 interacts with PSD-95 and regulates formation of dendritic spines. PMID: 24003235
  3. Cofilin activation during late cortical development depended on NOMA-GAP expression and subsequent inhibition of Cdc42. In utero expression of active cofilin was sufficient to restore postnatal dendritic complexity in NOMA-GAP-deficient animals. PMID: 22810622
  4. Overexpression of the full-length and C-terminal fragments of TCGAP inhibits insulin-stimulated glucose uptake and GLUT4 translocation. PMID: 12773384
Subcellular Location
Cell membrane. Note=Translocates to the plasma membrane in response to insulin in adipocytes.
Protein Families
PX domain-containing GAP family
Tissue Specificity
Highly expressed in brain and testis. Also expressed in white adipose tissue (WAT) and muscle at a low level.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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