Recombinant Mouse SET and MYND domain-containing protein 5 (Smyd5)

Code CSB-YP664920MO
MSDS
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Source Yeast
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Code CSB-EP664920MO
MSDS
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Source E.coli
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Code CSB-EP664920MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP664920MO
MSDS
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Source Baculovirus
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Code CSB-MP664920MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Smyd5
Uniprot No.
Alternative Names
Smyd5; Rai15; SET and MYND domain-containing protein 5; EC 2.1.1.-; Protein NN8-4AG; Retinoic acid-induced protein 15
Species
Mus musculus (Mouse)
Expression Region
1-416
Target Protein Sequence
MAASMCDVFS FCVGVADRAR GSVEVRYVDS IKGKGLFATQ LIRKGETIFI ERPLVAAQFL WNALYQYRAC DHCLRALEKA EENAQRLTGK PSQILPHPEL CSVRKDLHQN CPHCQVMYCS AECRLAAAEQ YHQILCPGPS HDPRHPLNKL QEAWRSVHYP PETASIMLMA RMVATVKQAK DKDHWVRLFN HFCSRTANQE QAIVHKLLKG KFKDQLELLL GLFKEALYEE ALSLWFTPEG FRSLFALVGT NGQGIGTSSL SQWVHACDAL ELTPQDREQL DTFIDQLYKD IEAATGEFLN CEGSGLFVLQ SCCNHSCVPN AETSFPENNF VLHVTALEDI KPGEEICISY LDCCQRERSR HSRHKILREN YLFNCSCPKC LAEADDPNVT SEEEEEEDEE EGEPEDAELG DEMTDV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4 to form trimethylated histone H4 lysine 20 (H4K20me3) which represents a specific tag for epigenetic transcriptional repression. In association with the NCoR corepressor complex, is involved in the repression of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages by catalyzing the formation of H4K20me3 at the gene promoters. Plays an important role in embryonic stem (ES) cell self-renewal and differentiation. Promotes ES cell maintenance by silencing differentiation genes through deposition of H4K20me3 marks. Maintains genome stability of ES cells during differentiation through regulation of heterochromatin formation and repression of endogenous repetitive DNA elements by depositing H4K20me3 marks.
Gene References into Functions
  1. Depletion of SMYD5 leads to compromised self-renewal, including dysregulated expression of OCT4 targets, and perturbed differentiation. PMID: 28250819
Protein Families
Class V-like SAM-binding methyltransferase superfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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