Recombinant Mouse Serine/threonine-protein kinase Nek11 (Nek11)

Code CSB-YP806004MO
MSDS
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Source Yeast
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Code CSB-EP806004MO
MSDS
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Source E.coli
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Code CSB-EP806004MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP806004MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP806004MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nek11
Uniprot No.
Alternative Names
Nek11; Serine/threonine-protein kinase Nek11; EC 2.7.11.1; Never in mitosis A-related kinase 11; NimA-related protein kinase 11
Species
Mus musculus (Mouse)
Expression Region
1-628
Target Protein Sequence
MLKFQETAKC VGRRPTVIPM YPTALIARRY VLQQKLGSGS FGTVYLVSDK KAKPGEELKV LKEISVGELN PNETVQANVE AQLLSRLHHP AIVRFHASFM EQETFCIITE YCEGRDLDYR IQEYKEAGKV FAENQIVEWF IQLLLGVDYM HERRILHRDL KSKNIFLKNN LLKIGDFGVS RLLMGSCELA TTLTGTPHYM SPEALKHQGY DAKSDIWSLA CILYEMCCLD HAFAGSSFLS VVLNIVEGKT PSLPDRYPRE LNTIMERMLN KSPSLRPSAA DILKAPYMEE QLQLLMCKYP EMTLEDKNSV CQKEAAHTIN AVQKKLHLQT LQALSDTQKT TPRERMWLRK LQAADERARR LKKIAEENYK ENDKRMQALR SRNVGSVHAH VLHELDERTL ESLPEPQSLP CLDLDELEPS LEDTIVDLGH YEIPEDPLVA EQYYSDVFDS CSEDSEEQEE EMIFSEAGGD TKEEESPSVY RTNQQDSDTA ALVGCLEHVL GYTSLDTKTI TNAVTDMSPG PMVFNSAVAR TKMKRMKESA VQKLGMETFE EVYDYLKRAR HQNAREAEIW EHLETVVPRA SDCFEVDQLL YFEELLLTME GKEPSLQNLP CEAAQKKPVK GTHFCDNP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.
Subcellular Location
Nucleus. Nucleus, nucleolus.
Protein Families
Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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