Recombinant Mouse Serpin A12 (Serpina12)

Code CSB-YP742491MO
MSDS
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Source Yeast
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Code CSB-EP742491MO
MSDS
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Source E.coli
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Code CSB-EP742491MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP742491MO
MSDS
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Source Baculovirus
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Code CSB-MP742491MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Serpina12
Uniprot No.
Alternative Names
Serpina12; Serpin A12; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin
Species
Mus musculus (Mouse)
Expression Region
21-413
Target Protein Sequence
LQDRDAPDMY DSPVRVQEWR GKKDARQLAR HNMEFGFKLL QRLASNSPQG NIFLSPLSIS TAFSMLSLGA QNSTLEEIRE GFNFKEMSNW DVHAAFHYLL HKLNQETEDT KMNLGNALFM DQKLRPQQRF LNLAKNVYDA DMVLTNFQDL ENTQKDINRY ISQKTHSRIK NMVKSIDPGT VMILTNYIYF RGRWQYEFDP KQTKEEEFFI EKGKTVKVPM MFQRGLYDMA YDSQLSCTIL EIPYRGNITA TFVLPDNGKL KLLEQGLQAD IFAKWKSLLS KRVVDVWVPK LRISSTYNMK KVLSRLGISK IFEENGDLTR ISSHRSLKVG EAVHKAELKM DEKGMEGAAG SGAQTLPMET PRHMKLDRPF LMMIYENFMP SMVFLARIYD PSG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
Gene References into Functions
  1. Vaspin mRNA and protein expression is increased in brown adipose tissue of cold-exposed and high-fat fed mice. PMID: 28580279
  2. Vaspin overexpression reduces ischaemia/reperfusion - induced myocardial apoptosis in mice. PMID: 29730286
  3. The results of the current study indicated that vaspin inhibited the progression of atherosclerotic plaques in apoE(/) mice by inhibiting endoplasmic reticulum stress-induced macrophage apoptosis. PMID: 26708512
  4. Vaspin inhibited RANKL-induced osteoclastogenesis in RAW264.7 cells and bone marrow cells. PMID: 23323745
  5. Vaspin is a novel ligand for the cell-surface GRP78/voltage-dependent anion channel complex in endothelial cells and promotes proliferation, inhibits apoptosis, and protects vascular injuries in diabetes mellitus. PMID: 23307819
  6. Data suggest that vaspin is ligand for cell-surface GRP78 (glucose-regulated protein 78kD)/MTJ-1 (DnaJ-like protein 1) complex; subsequent signal transduction benefits endoplasmic reticulum stress-induced metabolic dysfunctions as seen in obesity. PMID: 22837305
  7. may be the compensatory molecule in the pathogenesis of metabolic syndrome. (review) PMID: 18800627

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Subcellular Location
Secreted.
Protein Families
Serpin family
Tissue Specificity
Expressed in visceral adipose tissues.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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