Product Details

Purity

>85% (SDS-PAGE)

Target Names

Slc8b1

Uniprot No.

Q925Q3

Alternative Names

Slc8b1; Nckx6; Nclx; Slc24a6; Mitochondrial sodium/calcium exchanger protein; Na(+)/K(+)/Ca(2+)-exchange protein 6; Sodium/calcium exchanger protein; mitochondrial; Sodium/potassium/calcium exchanger 6; Solute carrier family 24 member 6; Solute carrier family 8 member B1

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Mitochondrial sodium/calcium antiporter that mediates sodium-dependent calcium efflux from mitochondrion, by mediating the exchange of 3 sodium ions per 1 calcium ion. Plays a central role in mitochondrial calcium homeostasis by mediating mitochondrial calcium extrusion: calcium efflux is essential for mitochondrial function and cell survival, notably in cardiomyocytes. Regulates rates of glucose-dependent insulin secretion in pancreatic beta-cells during the first phase of insulin secretion: acts by mediating efflux of calcium from mitochondrion, thereby affecting cytoplasmic calcium responses. Required for store-operated Ca(2+) entry (SOCE) and Ca(2+) release-activated Ca(2+) (CRAC) channel regulation: sodium transport by SLC8B1 leads to promote calcium-shuttling that modulates mitochondrial redox status, thereby regulating SOCE activity. Involved in B-lymphocyte chemotaxis. Able to transport Ca(2+) in exchange of either Li(+) or Na(+), explaining how Li(+) catalyzes Ca(2+) exchange. In contrast to other members of the family its function is independent of K(+).

Gene References into Functions

Results found that NCLX reduction/inhibition markedly suppressed chemotaxis while increasing the random migration of B lymphocytes. In addition, the contributions of NCLX to motility were observed only in B lymphocytes and not in T lymphocytes. PMID: 27328625
tamoxifen-induced deletion of Slc8b1 in adult mouse hearts causes sudden death, with less than 13% of affected mice surviving after 14 days; lethality correlated with severe myocardial dysfunction and fulminant heart failure PMID: 28445457
NCLX mediates the mitochondrial Na(+) influx and is tuned to sense the TTX-sensitive cytosolic Na(+) responses. PMID: 24719357
NCLX is a novel molecule to regulate automaticity of cardiomyocytes via modulating sarcoplasmic reticulum Ca(2+) handling. PMID: 24067497
Silencing of NCLX expression significantly reduces Ca2+-dependent processes in astrocytes. PMID: 23616530
The findings suggest that the mitochondrial Na(+)/Ca(2+) exchanger, NCLX, shapes glucose-dependent mitochondrial and cytosolic Ca(2+) signals thereby regulating the temporal pattern of insulin secretion in beta cells. PMID: 23056385
data provide evidence for the presence of an electrogenic Na(+)/Ca(2+) exchanger that is capable of regulating [Ca(2+)](i) after release of Ca(2+) from cell stores PMID: 11940515
Presence of a Na(+)-Ca(2+) exchanger in podocytes and its regulation by PKC. PMID: 12270979
Ca(2+) entering the myocyte via NCX can cause Ca(2+) sparks which are similar to those elicited by electrical stimulation. However, Ca(2+) influx on NCX is much less efficient in inducing Ca(2+) sparks than Ca(2+) influx via I(Ca,L). PMID: 12767888
An electrophysiologically distinct subset of arcuate nucleus neurons coexpress orexin receptors and glutamate decarboxylase-67 and are excited by orexin, activating a sodium-calcium exchange current, thereby depolarizing the cell and increasing its firing PMID: 12832517

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Subcellular Location

Mitochondrion inner membrane; Multi-pass membrane protein. Note=Mainly localizes to mitochondrion inner membrane (PubMed:20018762).; [Isoform 2]: Cell membrane; Multi-pass membrane protein.

Protein Families

Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family, SLC24A subfamily

Tissue Specificity

Ubiquitously expressed. Expressed in dental tissues.

Database Links

KEGG: mmu:170756

STRING: 10090.ENSMUSP00000064714

UniGene: Mm.291070

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Recombinant Mouse Sodium/potassium/calcium exchanger 6 (Slc24a6), partial

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