Recombinant Mouse Spermatid-specific linker histone H1-like protein (Hils1)

Code CSB-YP865596MO
MSDS
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Source Yeast
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Code CSB-EP865596MO
MSDS
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Source E.coli
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Code CSB-EP865596MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865596MO
MSDS
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Source Baculovirus
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Code CSB-MP865596MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Hils1
Uniprot No.
Alternative Names
Hils1Spermatid-specific linker histone H1-like protein; TISP64
Species
Mus musculus (Mouse)
Expression Region
1-170
Target Protein Sequence
MAQMVAGDQD AGTLWVPSQS ESQTESDIST QSLRKPTMSY VILKTLADKR VHNCVSLATL KKAVSITGYN MTHNTWRFKR VLQNLLDKGM IMHVTCCKGA SGSLCLCKER ALKSNHRAKR CQDRQKSQKP QKPGQRESEP CQLLLSSKKK NDQLFKGVRR VAKGNRHCHY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
DNA-binding protein that may be implicated in chromatin remodeling and/or transcriptional regulation during spermiogenesis, the process of spermatid maturation into spermatozoa.
Gene References into Functions
  1. HILS1 is implicated in chromatin remodeling during mammalian spermiogenesis. PMID: 12920187
  2. Hils1 may play an important role in the structural changes of spermatid nuclei, such as nuclear condensation, and gene regulation of haploid germ cell differentiation. PMID: 14636221
  3. 318-base pair (bp) 5'-proximal region, corresponding to first 70-bp proximal TATA-less promoter, and 248 bp of 5'-untranslated region is sufficient to confer testis- and spermatid-specific transcription and posttranscriptional control of mouse hils1 gene. PMID: 14695911
Subcellular Location
Nucleus. Chromosome.
Protein Families
Histone H1/H5 family
Tissue Specificity
Expressed exclusively in the testis by haploid germ cells (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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