Recombinant Mouse T-cell ecto-ADP-ribosyltransferase 1 (Art2a)

Code CSB-YP323818MO
MSDS
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Source Yeast
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Code CSB-EP323818MO
MSDS
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Source E.coli
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Code CSB-EP323818MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP323818MO
MSDS
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Source Baculovirus
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Code CSB-MP323818MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Art2a
Uniprot No.
Alternative Names
Art2a; Art2a-ps; Rt6-1; Rt6.1; T-cell ecto-ADP-ribosyltransferase 1; EC 2.4.2.31; ADP-ribosyltransferase 2a pseudogene; ADP-ribosyltransferase C2 and C3 toxin-like 2; ARTC2; Mono(ADP-ribosyl)transferase 2A; NAD(+) glycohydrolase; EC 3.2.2.5; T-cell NAD(P)(+)--arginine ADP-ribosyltransferase 1; T-cell differentiation marker Rt6 homolog 1; T-cell mono(ADP-ribosyl)transferase 1
Species
Mus musculus (Mouse)
Expression Region
21-258
Target Protein Sequence
LAVPFMLDMA PNAFDDQYEG CVEDMEKKAP QLLQEDFNMN EELKLEWEKA EIKWKEIKNC MSYPAGFHDF HGTALVAYTG NIHRSLNEAT REFKINPGNF HYKAFHYYLT RALQLLSDQG CRSVYRGTNV RFRYTGKGSV RFGHFASSSL NRSVATSSPF FNGQGTLFII KTCLGAHIKH CSYYTHEEEV LIPGYEVFHK VKTQSVERYI QISLDSPKRK KSNFNCFYSG STQAANVS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has both ADP-ribosyltransferase activity and thiol-dependent NAD(+) glycohydrolase activity.
Gene References into Functions
  1. NAD induces calcium elevation and astrocyte death by an ART2 and P2X7-mediated mechanism. PMID: 22781627
  2. ART2a is required for ADP-ribosylation of cell surface proteins PMID: 12370300
  3. CD38 controls the level of cell surface protein ADP-ribosylation by limiting the substrate availability for ART2. PMID: 15749861
  4. Transfer of a genetically disrupted CD38 allele into the autoimmune diabetes-prone NOD/Lt background accelerated diabetes onset in both sexes, whereas transfer of a disrupted ART2a/b complex had no effect. PMID: 16585549
  5. identify ART2.1 as a new candidate for linking autocrine/paracrine activation of inflammatory macrophages to the release of NAD, a critical intracellular metabolite PMID: 17947697
  6. Extracellular nicotinamide adenine dinucleotide (NAD) acts via ART2.1 to regulate P2X7 purinergic receptor-dependent inflammatory responses in murine macrophages. PMID: 19542469

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Involvement in disease
A subset of Rt6+ regulatory T-cells may confer protection to autoimmune disease, and failure to develop this subset may result in enhanced susceptibility for autoimmune disease.
Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor.
Protein Families
Arg-specific ADP-ribosyltransferase family
Tissue Specificity
Expressed in spleen, intestine and thymus.
Database Links

KEGG: mmu:11871

UniGene: Mm.439746

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