Recombinant Mouse Thioredoxin domain-containing protein 3 (Txndc3)

Code CSB-YP765139MO
MSDS
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Source Yeast
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Code CSB-EP765139MO
MSDS
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Source E.coli
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Code CSB-EP765139MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP765139MO
MSDS
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Source Baculovirus
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Code CSB-MP765139MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nme8
Uniprot No.
Alternative Names
Nme8; Sptrx2; Txndc3; Thioredoxin domain-containing protein 3; NME/NM23 family member 8; Spermatid-specific thioredoxin-2; Sptrx-2
Species
Mus musculus (Mouse)
Expression Region
1-586
Target Protein Sequence
MASKKREVQL QSVVNSQNLW DEMLLNKGLT VIDVYQAWCG PCKAVQSLFR KLKNELNEDE ILHFVVAEAD NIVTLQPFRD KCEPVFLFSL NGKIIAKIQG ANAPLINRKV ITLIDEERKI VAGEMDRPQY VEIPLVDAID EEYGEVQYES AAEVYNMAII KPDAVLMRKN IEVREKIAKE GFVIEIQENL ILPEEVVREF YTHIADQPDF EEFVVSMTNG LSCVLIVSQE DSEVIQEETL PQTDTEEEPG VLEEPHVRFA PVMIKKKRDS LQEYMDRQHM SDYCDVEDDA VKVSKLIDIL FPDFKTMKST NVQTTLALLH PDICEEEKDD VLNVIHNEGF TILMQRQIVL SEEEARTVCK IHENEEYFDN LIGHMTSNHS YVLALRRENG VEYWKTLIGP KTIEEAYASH PQSLCVQFAS GNFPTNQFYG SSSKAAAEKE IAHFFPPQST LALIKPHVTH KERMEILKTI KEAGFELTLM KEMHLTPEHA NKIYFKITGK DFYKNVLEVL SLGMSLVMVL TKWNAVAEWR RMVGPVDPEE AKLLSPESLR AKYGLDILRN AVHGASNFSE ASEIISNVFT EGNPEN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probably required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of fibrous sheath (FS) proteins occurs. May be involved in the reduction of disulfide bonds within the sperm FS components. In vitro, it has neither NDP kinase nor reducing activity on disulfide bonds.
Gene References into Functions
  1. Txndc2 and Txndc3 are critically important in protecting spermatozoa against the increases in oxidative stress associated with paternal age. PMID: 23707457
  2. data indicate that spermatid-specific thioredoxin-2 incorporation into the sperm fibrous sheath(FS) lags well behind FS assembly, suggesting it is required during the final stages of sperm tail maturation PMID: 12909633
Subcellular Location
Cytoplasm.
Protein Families
NDK family
Tissue Specificity
Testis-specific. Expressed mainly in round spermatids.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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