Product Details

Purity

>85% (SDS-PAGE)

Target Names

Hes7

Uniprot No.

Q8BKT2

Alternative Names

Hes7; Transcription factor HES-7; mHes7; Hairy and enhancer of split 7; bHLH factor Hes7

Species

Mus musculus (Mouse)

Expression Region

1-225

Target Protein Sequence

MVTRERAENR DGPKMLKPLV EKRRRDRINR SLEELRLLLL ERTRDQNLRN PKLEKAEILE FAVGYLRERS RVEPPGVPRS PGQDAEALAS CYLSGFRECL LRLAAFAHDA SPAARSQLFS ALHGYRRPKP PRPEAVDPGL PAPRPPLDPA SPILGPALHQ RPPVHQGPPS PRLAWSPSHC SSRAGDSGAP APLTGLLPPP PPPYRQDGAP KAPSLPPPAF WRPWP

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Transcriptional repressor. Represses transcription from both N box- and E box-containing promoters. May with HES1, cooperatively regulate somite formation in the presomitic mesoderm (PSM). May function as a segmentation clock, which is essential for coordinated somite segmentation.

Gene References into Functions

Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis PMID: 29437832
Lengthening the third Hes7 intron by 10 or 20 kb disrupts accurate RNA splicing and inactivates the gene. PMID: 22326607
Fgf signaling was found to be a primary target for hypoxia, which causes phenotypic variations of heterozygous mutations in Hes7 or Mesp2, suggesting gene-environment interaction through this signaling. PMID: 23465881
Hes7 is a key regulator of the pace of the segmentation clock. PMID: 23219549
Tbx6 and the Wnt pathway cooperatively regulate proper Hes7 expression. PMID: 23326414
introns lead to an approximately 19-min delay in the Hes7 gene expression, and mathematical modeling suggested that without such a delay, Hes7 oscillations would be abolished PMID: 21300886
there are differential axial requirements for lunatic fringe and Hes7 transcription during mouse somitogenesis PMID: 19956724
periodic repression by Hes7 protein is critical for the cyclic transcription of Hes7 and Lfng, and this negative feedback represents a molecular basis for the segmentation clock PMID: 12783854
instability of Hes7 is essential for sustained oscillation and for its function as a segmentation clock PMID: 15170214
Lunatic fringe and Hes7 controls their oscillatory expression during somitogenesis by forming negative feedback loop PMID: 16342160
Hes7 oscillation is initiated by Fgf signaling and propagated/maintained anteriorly by Notch signaling. PMID: 17681139
repressor functionality and the half-life of the protein is crucial for oscillatory expression of Hes7 and regular somite formation PMID: 17983837
These results indicate that common amino acid residues are involved in both the transcriptional repressor activity and the instability of Hes7 protein, suggesting of a critical link between the transcription and degradation control. PMID: 18477475
Sprouty4 does not oscillate in Hes7-null mutant mouse embryos, and Hes7 can inhibit FGF-induced transcriptional activity of the Sprouty4 promoter. PMID: 19440349

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Subcellular Location

Nucleus.

Database Links

KEGG: mmu:84653

STRING: 10090.ENSMUSP00000024543

UniGene: Mm.98505

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Recombinant Mouse Transcription factor HES-7 (Hes7)

Product Details

Customer Reviews and Q&A

Target Background

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