Recombinant Mouse Transcription termination factor, mitochondrial (Mterf)

Code CSB-YP811872MO
MSDS
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Source Yeast
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Code CSB-EP811872MO
MSDS
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Source E.coli
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Code CSB-EP811872MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP811872MO
MSDS
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Source Baculovirus
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Code CSB-MP811872MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mterf1a
Uniprot No.
Alternative Names
Mterf1a; Mterf; Mterf1; Transcription termination factor 1a; mitochondrial; Mitochondrial transcription termination factor 1a; mTERF1a
Species
Mus musculus (Mouse)
Expression Region
38-379
Target Protein Sequence
FSA ECDSKDKESL EEEREDLLSN LVTMGVDIDM ARRRQPGVFN KAVTNEQELK LFLLSKGASD KVIGSIISRY PRAITRTPES LSKRWDLWRK IMASDLEIVN ILERSPESFF RSNNNLNLEN NIKFLCSVGL THKCLCRLLT NAPRTFSNSL NLNKQMVEFL QETGMSLGHN DPRDFVRKII SKNPSILIQS TKRVKTNIEF LQSTFNLNKR DLLLLICGPG ARILDLSNDC TKKNYTNIRE RLLSLGCSEE EVQRFVLSYL NMVFLSEKKF NDKIDCLIEE KISASQIIEN PRILDSSINT LKTRIRELSH AGYDLSTSSI ALLSWSQRRY EAKLKRLCG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Transcription termination activity appears to be polarized with highest specificity for transcripts initiated on the light strand.
Gene References into Functions
  1. MTERF1 does not regulate heavy-strand transcription, but rather acts to block transcription on the opposite strand of mtDNA to prevent transcription interference at the light-strand promoter. PMID: 23562081
Subcellular Location
Mitochondrion.
Protein Families
MTERF family
Tissue Specificity
Predominantly expressed in heart and liver, with extremely low levels in other tissues. Expressed strongly in the heart and at lower levels in brain, liver and kidney.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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