Recombinant Mouse Transcriptional repressor CTCF(Ctcf),partial

Code CSB-YP726738MO1
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Source Yeast
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Code CSB-EP726738MO1
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Source E.coli
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Code CSB-EP726738MO1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP726738MO1
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Source Baculovirus
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Code CSB-MP726738MO1
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Ctcf
Uniprot No. Q61164
Alternative Names CtcfTranscriptional repressor CTCF; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping (By similarity). Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X-inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis (By similarity).
Gene References into Functions
  1. Data suggest that CTCF sites are porous borders, allowing a super-enhancer to activate a secondary target. Likewise, CTCF sites shield a widely expressed gene from suppressive influences of a silent locus. PMID: 28714474
  2. maintains the germinal center transcriptional programme, allows a high proliferation rate, and represses the expression of Blimp-1, the master regulator of plasma cell differentiation PMID: 28677680
  3. Dxz4 represents a structural platform for frequent long-range contacts with multiple loci in a direction dictated by the orientation of its bank of CTCF motifs, which may work as a ratchet to form the distinctive bipartite structure of the condensed inactive X chromosome PMID: 29654302
  4. neither the deletion of the CTCF locus nor the ectopic insertion of Firre cDNA or its ectopic expression are sufficient to alter topologically associated domains in a sex-specific or allele-specific manner PMID: 29654311
  5. CTCF maintains regulatory homeostasis of cancer pathways. PMID: 30086769
  6. CTCF plays an essential role in otic neurogenesis by modulating histone modification in the Neurog1 locus. PMID: 30008200
  7. CTCF and BORIS cooperate with additional Testis-specific transcriptional regulators to regulate gene expression in developing male gametes. PMID: 28145452
  8. CCCTC-binding factor CTCF supports the homeostatic maintenance of hematopoietic stem cell (HSCs) pools by sustaining HSC quiescence in a reactive oxygen species ROS-dependent manner. PMID: 28857086
  9. Authors show that mice devoid of an inducible CTCF binding element, located in the alpha constant gene, display a marked isotype-specific increase of GL transcription in developing and resting splenic B cells and altered CSR in activated B cells. PMID: 28533409
  10. The study provides evidence that CTCF regulates chromatin organization during spermiogenesis, contributing to the functional organization of mature sperm. PMID: 27345455
  11. CTCF Binding Sites in the Herpes Simplex Virus 1 Genome Display Site-Specific CTCF Occupation, Protein Recruitment, and Insulator Function. PMID: 29437965
  12. Both LDB1 and CTCF are required for enhancer-Car2 looping, and the domain of LDB1 contacted by CTCF is necessary to rescue Car2 transcription in LDB1-deficient cells. PMID: 28636938
  13. CTCF physically associates with Wdr5 and further transcriptionally controls its expression by directly targeting the Wdr5 gene promoter. PMID: 28193136
  14. Since CTCF and cohesin are required for loop domain formation, our results suggest that chromatin loops are dynamic and frequently break and reform throughout the cell cycle. PMID: 28467304
  15. Chromatin immunoprecipitation-DNA sequence analysis was performed in adult cerebellum and Wiz peaks were found at promoters and transcription factor CTCF binding sites. PMID: 27410475
  16. CTCF mediates local regulatory interactions to coordinate transcriptional programs controlling transitions in morphology and function during heart development. PMID: 28846746
  17. Long-range promoter-enhancer interaction mediated by CTCF plays important roles in controlling the cell-to-cell variation of gene expression in mammalian cells. PMID: 28938092
  18. n keratinocytes, the promoter-enhancer anchoring regions in the gene-rich transcriptionally active TADs are enriched for the binding of chromatin architectural proteins CTCF, Rad21 and chromatin remodeler Brg1. In contrast to gene-rich TADs, gene-poor TADs show preferential spatial contacts with each other, do not contain active enhancers and show decreased binding of CTCF, Rad21 and Brg1 in keratinocytes PMID: 28863138
  19. CTCF mediated chromatin structural modulation and regulates Hoxc gene expression. PMID: 27080371
  20. CTCF can regulate V(D)J recombination by segregating recombination signal sequences (RSSs) into distinct loop domains and inhibiting RSS synapsis, independent of any effects on transcription, RSS accessibility, and RAG recombinase tracking PMID: 27613698
  21. this study shows that CTCF serves to translate alpha-ketoglutarate-sensitive metabolic changes into context-dependent differentiation gene programs PMID: 28813658
  22. CTCF-cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo. PMID: 28737770
  23. ERCC1-XPF cooperates with CTCF and cohesin to facilitate the developmental silencing of imprinted genes and that persistent DNA damage triggers chromatin changes that affect gene expression programs associated with NER disorders. PMID: 28368372
  24. BRD2 acts to augment the boundary function of CTCF both by limiting the spread or range of enhancer activity and by physically preventing the formation of cross-boundary contacts genome-wide. PMID: 28388437
  25. Functional assessment of CTCF sites at cytokine-sensing mammary enhancers using CRISPR/Cas9 gene editing in mice has been reported. PMID: 28334928
  26. distribution of cohesin in the mouse genome depends on transcription, CTCF and the cohesin release factor Wings apart-like (Wapl) PMID: 28424523
  27. deletion of CTCF (CCCTC-binding factor)-binding sites at TAD and sub-TAD topological boundaries that form within the HoxA and HoxC clusters during differentiation not only disturbs local chromatin domain organization and regulatory interactions but also results in homeotic transformations typical of Hox gene misregulation PMID: 28087711
  28. Our study also identified the imprinting control region (ICR) of H19 as a genomic target. According to the results, PEG3 binds to a specific sequence motif located between the 3(rd) and 4(th) CTCF binding sites of the H19-ICR. PEG3 also binds to the active maternal allele of the H19-ICR. PMID: 27824289
  29. data demonstrate a reciprocal relationship between Hmgb2 and Ctcf in controlling aspects of chromatin structure and gene expression. PMID: 27226577
  30. in addition to mediating long-range chromatin interactions, CTCF influences intricate configuration of chromatin loops that govern functional interactions between elements. PMID: 28137913
  31. TGF-beta/beta2-spectrin/CTCF-regulated tumor suppression in human stem cell disorder Beckwith-Wiedemann syndrome. PMID: 26784546
  32. Results indicate that CTCF binding polarity plays a functional role in the formation of higher-order chromatin structure. PMID: 26527277
  33. Firre is X-linked and expressed from a macrosatellite repeat locus associated with a cluster of CTCF and cohesin binding sites, and is preferentially located adjacent to the nucleolus PMID: 25887447
  34. CTCF controls the homeostatic maintenance and migration of Langerhans cells. PMID: 25936568
  35. MyoD affects chromatin looping at CCCTC-binding factor-binding sites represents the first evidence that a differentiation factor regulates chromatin-loop dynamics PMID: 25002401
  36. Findings establish CTCF as a prominent tumor-suppressor gene and point to CTCF-mediated epigenetic stability as a major barrier to neoplastic progression. PMID: 24794443
  37. CTCF is recruited in a locus-specific manner and CTCF-RNA interactions may be involved in long-range chromosomal interactions. PMID: 25578877
  38. TET1/DNA methylation-dependent nucleosome repositioning is the main mechanism that drives differential CTCF binding site selection during stem cell development. PMID: 24812327
  39. NURF regulation occurs partly through physical and functional interactions with the ubiquitous and multivalent factors Ctcf and cohesin PMID: 25348714
  40. CTCF is involved in regulating endocrine function of pancreatic islet cells by suppression of Pax6 expression. PMID: 24806748
  41. Studies show that cohesin associates with active enhancers and promoters and with CTCF to form long-range interactions important for gene regulation. PMID: 24002784
  42. STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells. PMID: 24711622
  43. B cell differentiation is associated with reprogramming the CCCTC binding factor-dependent chromatin architecture of the murine MHC class II locus. PMID: 24634495
  44. Th17 lineage differentiation is restrained by the Th2 locus via interchromosomal associations organized by Oct-1 and CTCF. PMID: 24613343
  45. non-coding RNA transcription could be the cause and the consequence of CTCF eviction. PMID: 23933634
  46. CTCF activities are required for two distinct events in early cortex formation: first, to correctly regulate the balance between neuroprogenitor cell proliferation and differentiation, and second, for the survival of neuroprogenitor cells PMID: 24553927
  47. Findings link CTCF and cohesin with the control regions of a subset of imprinted genes, supporting the notion that imprinting control is mechanistically diverse. PMID: 23804403
  48. CTCF functioned as a molecular mediator between insulin-induced upstream Erk signalling and Pax6 expression in pancreatic beta-cells. PMID: 24354619
  49. CTCF associates with a wide array of DNA modules via combinatorial clustering of its 11 zinc fingers. PMID: 23707059
  50. a direct regulatory relationship between EGF-induced NFkappaB p50 and CTCF activation affecting corneal epithelial wound healing has been established PMID: 23843455

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Subcellular Location Nucleus, nucleoplasm, Chromosome, Chromosome, centromere
Protein Families CTCF zinc-finger protein family
Database Links

KEGG: mmu:13018

STRING: 10090.ENSMUSP00000005841

UniGene: Mm.269474

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