Product Details

Purity

>85% (SDS-PAGE)

Target Names

Myo1c

Uniprot No.

Q9WTI7

Alternative Names

Myo1c; Unconventional myosin-Ic; Myosin I beta; MMI-beta; MMIb

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which then are moved relative to actin filaments. Involved in glucose transporter recycling in response to insulin by regulating movement of intracellular GLUT4-containing vesicles to the plasma membrane. Component of the hair cell's (the sensory cells of the inner ear) adaptation-motor complex. Acts as a mediator of adaptation of mechanoelectrical transduction in stereocilia of vestibular hair cells. Binds phosphoinositides and links the actin cytoskeleton to cellular membranes.; Isoform 3 is involved in regulation of transcription. Associated with transcriptional active ribosomal genes. Appears to cooperate with the WICH chromatin-remodeling complex to facilitate transcription. Necessary for the formation of the first phosphodiester bond during transcription initiation.

Gene References into Functions

Myo1c ensembles can generate forces parallel to lipid bilayers. PMID: 27156719
We propose a novel genome-wide mechanism where myosin synergizes with Pol II-associated actin to link the polymerase machinery with permissive chromatin for transcription activation. PMID: 26044184
Our observations demonstrate specific changes in the expression of myosin IC isoform A that are concurrent with the occurrence of prostate cancer in the TRAMP mouse prostate cancer model that closely mimics clinical prostate cancer PMID: 25259793
Ca(2+) binding to calmodulin induces major conformational changes in both IQ motifs and the post-IQ domain and increases flexibility of the myosin-1c tail. PMID: 25437912
The v-Crk-myosin-1c interaction, which modulates membrane dynamics by regulating Rac1 activity, is crucial for cell adhesion and spreading. PMID: 23983611
Mouse nuclear myosin I knock-out shows interchangeability and redundancy of myosin isoforms in the cell nucleus. PMID: 23593477
Myo1c functions as a slow transporter rather than a tension-sensitive anchor. PMID: 22908250
the novel specific NLS brings to the cell nucleus not only the "nuclear" isoform of myosin I (NM1 protein) but also its "cytoplasmic" isoform (Myo1c protein) PMID: 22295092
The data suggest that Myosin 1c is involved in the cytoskeleton dynamics and membrane protein anchoring or sorting in B lymphocytes PMID: 21841128
A hearing loss-associated myo1c mutation (R156W) decreases the myosin duty ratio and force sensitivity PMID: 21265502
The strength and attachment lifetime of single myo1c molecules as they bind beads coated with a bilayer of 2% phosphatidylinositol 4,5-bisphosphate and 98% phosphatidylcholine, were measured. PMID: 21156133
Myo1c is a novel mediator of both insulin-stimulated and contraction-stimulated glucose uptake in skeletal muscle. PMID: 21127070
Mutations in the Myo1c gene affect the interaction of Myo1c with nucleotides and actin. PMID: 20640478
myosin Myo1c functions in a PI(3)K-independent insulin signalling pathway that controls the movement of intracellular GLUT4-containing vesicles to the plasma membrane PMID: 12490950
localized membrane remodeling driven by the Myo1c motor appears to facilitate the fusion of exocytic GLUT4-containing vesicles with the adipocyte plasma membrane PMID: 15169906
These data are consistent with Myo1c participating in the regulation of the Na+ channel after antidiuretic hormone stimulation. PMID: 15716323
These results suggest that mechanical activity of myosin-1c is required for fast adaptation in vestibular hair cells. PMID: 16102537
myo1c does not bind to physiological concentrations of phosphatidylserine but rather binds tightly to phosphatidylinositol 4,5-bisphosphate PMID: 16492791
Motor protein Myo1c and its receptor protein NEMO act cooperatively to form the IKK-IRS-1 complex and function in TNF-alpha-induced insulin resistance. PMID: 16754954
Subcellular localization of class I and class II myosin during B cell spreading showed differences, following CD44 cross-linking, myosin-1c was polarized to lamellipodia while the distribution of cytosplasmic nonmuscle class II myosin was not altered. PMID: 16919270
Two distinct membrane binding sites within the regulatory and tail domains of myo1c protein were characterized. PMID: 16971510
Calcium regulation of calmodulin binding to and dissociation from the myo1c regulatory domain was studied. PMID: 17910470
These findings suggest that the Rictor-Myo1c complex is distinct from mTORC2 and that Myo1c, in conjunction with Rictor, participates in cortical actin remodeling events. PMID: 18426911
insulin regulates Myo1c function via CaMKII-dependent phosphorylation, and these events play a role in insulin-regulated GLUT4 trafficking in adipocytes likely involving Myo1c motor activity. PMID: 19046570
phosphoinositides are able to bind to and dissociate from myo1c(IQ-tail) as it remains bound to the membrane PMID: 19706607

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Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, stereocilium membrane. Cytoplasmic vesicle. Cell projection, ruffle.

Protein Families

TRAFAC class myosin-kinesin ATPase superfamily, Myosin family

Tissue Specificity

Isoform 3 is expressed in small intestine, pancreas, brain, kidney, skin, heart muscle, testis, striated muscle, spleen, liver and lung (at protein level). Expressed in brain, testis, adrenal glands, thymus, spleen, kidney, lung, heart, cochlea and vestib

Database Links

KEGG: mmu:17913

STRING: 10090.ENSMUSP00000104069

UniGene: Mm.234502

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Recombinant Mouse Unconventional myosin-Ic (Myo1c), partial

Product Details

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Target Background

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