Recombinant Mouse Unconventional myosin-VIIa (Myo7a), partial

Code CSB-YP015352MO
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP015352MO
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP015352MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP015352MO
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP015352MO
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Myo7a
Uniprot No.
Alternative Names
Myo7a; Myo7; Unconventional myosin-VIIa
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. Mediates intracellular transport of RPE65 in the retina pigment epithelium. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity.
Gene References into Functions
  1. Many of the genes found in the genetic network, pathways, and gene ontology categories of Myo7a are related to either deafness or blindness in Usher syndrome mouse model. PMID: 29430167
  2. Here, the authors show that exophilin-8 accumulates granules in the cortical F-actin network not by direct interaction with myosin-Va, but by indirect interaction with a specific form of myosin-VIIa through its previously unknown binding partner, RIM-BP2. PMID: 28673385
  3. MYO7A and PDZD7 interact in tissue-culture cells, and co-localize to the ankle-link region of stereocilia in wild-type but not Myo7a mutant mice. PMID: 27525485
  4. MYO7A binds to and impinges on CASPASE-8, revealing a new regulatory axis affecting RIPK1>CASPASE-8 signaling. Results expose a conserved role for unconventional myosins in transducing caspase-dependent regulation of kinases. PMID: 26960254
  5. Data show that myosin7a (Myo7a; sh1) deficiency causes severe retinal dysfunctions in albino sh1-/- mice. PMID: 23991031
  6. The family was found to segregate novel mutations of two different genes: myosin VIIA (MYO7A), and phosphodiesterase 6B, which causes nonsyndromic retinitis pigmentosa. PMID: 23882135
  7. the importance of MYO7A for the development and maintenance of bundle function PMID: 22381527
  8. the results support a role for MYO7A in the translocation of RPE65, illustrating the involvement of a molecular motor in the spatiotemporal organization of the retinoid cycle in vision. PMID: 21493626
  9. the myosin VIIa is a "slow", monomeric molecular motor with a duty ratio of 0.6. PMID: 21212272
  10. crystal structure of MYO7A MyTH4-FERM domains in complex with the central domain (CEN) of Sans at 2.8 angstrom resolution; MyTH4-FERM/CEN complex structure provides mechanistic explanations for known deafness-causing mutations in MYO7A MyTH4-FERM PMID: 21311020
  11. Cadherin-23, myosin VIIa and harmonin form a ternary complex and interact with phospholipids. PMID: 20639393
  12. MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear PMID: 19774077
  13. The carboxy-terminal FERM domain of myosin VIIA is critical for melanosome transport in retinal pigment epithelial cells. PMID: 20016096
  14. participates in anchoring and holding membrane-bound elements to the actin core of the stereocilium PMID: 11753415
  15. Cdh23 and Myo7a are both required for establishing and/or maintaining the proper organisation of the stereocilia bundle and that they do not genetically interact to affect this process nor to cause age-related hearing loss. PMID: 12121736
  16. Slac2-c interacts with Rab27, actin, myosin Va and this protein PMID: 12221080
  17. the shaping of the hair bundle relies on a functional unit composed of myosin VIIa, harmonin b and cadherin 23 that is essential to ensure the cohesion of the stereocilia PMID: 12485990
  18. Noise-induced hearing loss in 11-12-week-old mice heterozygous for a null allele of Myo7a ( Myo7a(4626SB)) is not significantly different from wild-type littermates. PMID: 14648237
  19. A Myo7a mutation cosegregates with stereocilia defects and low-frequency hearing impairment. PMID: 15389316
  20. Slac2-c acts as a functional myosin VIIa receptor and the Rab27A.Slac2-c x myosin VIIa tripartite protein complex regulates the transport of retinal melanosomes in pigment epithelium cells PMID: 15927964
  21. Protocadherin 15 and myosin 7a cooperate to regulate the development and function of the mechanically sensitive hair bundle. PMID: 16481439
  22. The present results provide evidence from live retinal pigment epithelium cells that the RAB27A-MYRIP-MYO7A complex functions in melanosome motility. PMID: 17352418
  23. Shroom2 and ZO-1 form a tight-junction-associated scaffolding complex, possibly linked to myosin VIIa, that bridges the junctional membrane to the underlying cytoskeleton, thereby contributing to the stabilization of these junctions. PMID: 17666436
  24. Mosaic complementation demonstrates a regulatory role for Myo7a in actin dynamics of stereocilia. PMID: 18160714
  25. The data indicate that melanosomes in the retinal pigment epithelium and choroid are the dominant source of NIR-autofluorescence from the posterior region of the eye with ushers syndrome due to MYO7A mutation. PMID: 19324852
  26. A MYO7A-MYRIP-RAB27A complex is needed for apical melanosome tethering and regulates transport in RPE (retinal pigment epithelium) cells. MYRIP recruitment to the apical RPE is independent of RAB27A or melanosomes. PMID: 17352418
  27. Functional replacement with human MYO7A using lentiviral vectors rescues defects in opsin transport, melanosome transport and degradation of rod outer segment membranes in the retina's of shaker1 mice null for Myo7a PMID: 17268537
  28. myosin VIIa is required for the correct transport and degradation of ingested photoreceptor outer segment membranes by the RPE (retinal pigmented epithelium) PMID: 12743369
  29. myosin VIIa and Rab27a regulate the transport and tethering of RPE (retinal pigmented epithelium) melanosomes PMID: 15572405
  30. Shown to be a functional mechanoenzyme with actin activated ATPase activity as well as being a calcium sensitive calmodulin binding protein. PMID: 11839794

Show More

Hide All

Involvement in disease
Defects in Myo7a are the cause of the shaker-1 (sh-1) phenotype which affects only the inner ear. Sh-1 homozygote mutants show hyperactivity, head tossing and circling due to vestibular dysfunction, together with typical neuroepithelial-type cochlear defects involving dysfunction and progressive degeneration of the organ of Corti.
Subcellular Location
Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell junction, synapse.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
Tissue Specificity
Detected in mechanosensory stereocilia of cochlea hair cells (at protein level). Expressed in the retina, cochlea, kidney and liver.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1