Recombinant Mouse Urocortin-2 (Ucn2)

Code CSB-YP858154MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP858154MO
MSDS
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Source E.coli
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Code CSB-EP858154MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP858154MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP858154MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ucn2
Uniprot No.
Alternative Names
Ucn2; Urocortin-2; Urocortin II; Ucn II
Species
Mus musculus (Mouse)
Expression Region
73-110
Target Protein Sequence
VILSLDVP IGLLRILLEQ ARYKAARNQA ATNAQILAHV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress.
Gene References into Functions
  1. Urocortin 2 acts on gonadotrophs expressing the corticotropin-releasing factor type 2 receptor, and inhibits the production of gonadotropins in the pituitary gonadotropic tumor cells. PMID: 28851616
  2. Co-administration of neutralizing interleukin-6 receptor antibodies (0.2 mg kg(-1) ) and corticotrophin-releasing factor receptor 2 agonist. PMID: 28665499
  3. results indicate that long-term UVA eye irradiation led to increased gp91phox-derived ROS in the brain and the increased expression of urocortin 2 and CRHR type 2, resulting in photoaging; however, further studies are needed to confirm these findings PMID: 26648475
  4. increased significantly in placenta approaching birth PMID: 25426872
  5. Urocortin 2 autocrine/paracrine and pharmacologic effects to activate AMP-activated protein kinase in the heart. PMID: 24043794
  6. Description of ucn2 posttranslational processing and peptide sequence. Hypoxia and glycosylation, paradigms that might influence secretion or processing of gene products, did not significantly impact hUcn 2 prohormone cleavage. PMID: 23493376
  7. this study demonistrated that male UCN2 null mice showed more passive social interactions. PMID: 22640813
  8. Ucn2 can modulate GnRH mRNA levels PMID: 22138165
  9. CRF(1) agonists, Ucn 1 and stressin(1) -A, reduced feeding and induced interoceptive stress, whereas Ucn 2 potently suppressed feeding via a CRF(2) -dependent mechanism without eliciting malaise PMID: 21627635
  10. findings suggest an important role for UCN2 in cardiovascular homeostasis PMID: 19914944
  11. Ucn II is expressed in the hypothalamus, brainstem, olfactory bulb, and pituitary. Low levels were also detected in the mouse cortex, hippocampus, and spinal cord, Ucn II gene transcription was stimulated by glucocorticoids PMID: 12764078
  12. significant increase in urocortin II mRNA levels in the skin, but not in skeletal muscle, of both corticotropin-releasing factor receptor 1 and 2-null mice PMID: 14736736
  13. Urocortin 2 induces macrophage apoptosis via Corticotropin-Releasing Factor Receptor-2. PMID: 16054139
  14. The differential phenotype of urocortin 2 deficiency in female and male mice may imply a role for urocortin 2 in these gender differences. PMID: 16707802
  15. Ucn 2 inhibited insulin-induced Akt and ERK1/2 phosphorylation in cultured skeletal muscle cells and C2C12 myotubes PMID: 17050686
  16. The selective CRF2 agonist prevented enhanced defecation. PMID: 17194724
  17. the Ucn-CRH receptor system may be regulated by two major forms of cardiac stresses, i.e. oxidative and inflammatory stress, and may play a critical role in cardiac stress adaptation in heart diseases PMID: 19318426
  18. Urocortin 2 inhibits furosemide-induced activation of renin and enhances renal function and diuretic responsiveness in experimental heart failure. PMID: 19919977

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Subcellular Location
Secreted.
Protein Families
Sauvagine/corticotropin-releasing factor/urotensin I family
Database Links
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