Recombinant Mouse Zinc finger X-chromosomal protein (Zfx)

Code CSB-YP026467MO
MSDS
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Source Yeast
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Code CSB-EP026467MO
MSDS
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Source E.coli
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Code CSB-EP026467MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026467MO
MSDS
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Source Baculovirus
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Code CSB-MP026467MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Zfx
Uniprot No.
Alternative Names
ZfxZinc finger X-chromosomal protein
Species
Mus musculus (Mouse)
Expression Region
1-799
Target Protein Sequence
MDEDGLELQQ QAPNSFFDAT GAGATHMDGN QIVVEVQETV YVSDVVDSDI TVHNYVPDDP DSVVIQDVIE DVVIEDVQCT DIMDEADVSE TVIIPEQVLD SDVTEEVSLT HCTVPDDVLA SDITSASISM PEHVLTSESI HVSDVGHVEH VVHDSVVEAE IVTDPLAADV VSEEVLVADC ASEAVIDANG IPVNQQDEEK NNCEDYLMIS LDDAGKIEHD GSSGLTMDNE TEIDPCKVDG TCPEVIKVYI FKADPGEDDL GGTVDIVESE PENEHGVELL DPNNSIRVPR EKMVYMAVND SQQEEEELNV AEIADEVYME VIVGEEDAAA AAAAAVHEQQ VEDNEMKTFM PIAWAAAYGN NSDGIENRNG TASALLHIDE SAGLGRLAKQ KPKKRRRPDS RQYQTAIIIG PDGHPLTVYP CMICGKKFKS RGFLKRHMKN HPEHLAKKKY RCTDCDYTTN KKISLHNHLE SHKLTSKAEK AIECDECGKH FSHAGALFTH KMVHKEKGAN KMHKCKFCEY ETAEQGLLNR HLLAVHSKNF PHICVECGKG FRHPSELKKH MRIHTGEKPY ECQYCEYRSA DSSNLKTHVK TKHSKEMPFK CDICLLTFSD TKEVQQHALV HQESKTHQCL HCDHKSSNSS DLKRHIISVH TKDYPHKCDM CDKGFHRPSE LKKHVAAHKG KKMHQCRHCD FKIADPFVLS RHILSVHTKD LPFRCKRCRK GFRQQSELKK HMKTHSGRKV YQCEYCEYST TDASGFKRHV ISIHTKDYPH RCEYCKKGFR RPSEKNQHIM RHHKEVGLP
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable transcriptional activator.
Gene References into Functions
  1. our findings show that RNAi-mediated disruption of Zfx/Zfy in mouse testis affected X/Y spermatogenesis. Additionally, results suggest that the paralogous genes Zfx/Zfy play an important role in the process of X and Y sperm development. The individual interference of Zfx/Zfy may predict the outcome of X and Y haploid sperms. Presented herein is an advanced method developed to control mouse X/Y spermatogenesis and sex rati PMID: 28251288
  2. Zfx is a common cell-intrinsic regulator of diverse Hedgehog-induced tumors. PMID: 25164012
  3. Zfx, a transcription factor that controls hematopoietic stem cell self-renewal, controls the initiation and maintenance of acute myeloid leukemia. PMID: 24485662
  4. These studies identify Zfx as a shared transcriptional regulator of embryonic stem cells and hematopoietic stem cells, suggesting a common genetic basis of self-renewal in embryonic and adult SC. PMID: 17448993
  5. Zfx restrains the stress response and couples antigen receptor signaling to cell expansion and maintenance during B-cell development and peripheral homeostasis PMID: 19329779

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Subcellular Location
Nucleus.
Protein Families
Krueppel C2H2-type zinc-finger protein family, ZFX/ZFY subfamily
Database Links

KEGG: mmu:22764

STRING: 10090.ENSMUSP00000085423

UniGene: Mm.919

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