Recombinant Mouse Zinc finger protein Aiolos (Ikzf3)

Code CSB-YP011577MO
MSDS
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Source Yeast
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Code CSB-EP011577MO
MSDS
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Source E.coli
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Code CSB-EP011577MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011577MO
MSDS
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Source Baculovirus
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Code CSB-MP011577MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ikzf3
Uniprot No.
Alternative Names
Ikzf3; Zfpn1a3; Znfn1a3; Zinc finger protein Aiolos; Ikaros family zinc finger protein 3
Species
Mus musculus (Mouse)
Expression Region
1-507
Target Protein Sequence
MEDIQPTVEL KSTEEQPLPT ESPDALNDYS LPKPHEIENV DSREAPANED EDAGEDSMKV KDEYSDRDEN IMKPEPMGDA EESEMPYSYA REYSDYESIK LERHVPYDNS RPTGGKMNCD VCGLSCISFN VLMVHKRSHT GERPFQCNQC GASFTQKGNL LRHIKLHTGE KPFKCHLCNY ACQRRDALTG HLRTHSVEKP YKCEFCGRSY KQRSSLEEHK ERCRAFLQNP DLGDAASVEA RHIKAEMGSE RALVLDRLAS NVAKRKSSMP QKFIGEKRHC FDANYNPGYM YEKENEMMQT RMMDQAINNA ISYLGAEALR PLVQTPPAPT SEMVPVISSV YPIALTRADM PNGAPQEMEK KRILLPEKIL PSERGLSPNN SAQDSTDTDS NHEDRQHLYQ QSHVVLPQAR NGMPLLKEVP RSFELLKPPP ICLRDSIKVI NKEGEVMDVF RCDHCHVLFL DYVMFTIHMG CHGFRDPFEC NMCGYRSHDR YEFSSHIARG EHRAMLK
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Binds to GGGAA. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
Gene References into Functions
  1. These data place Aiolos in the emerging gene regulatory network controlling NK-cell maturation and function. PMID: 25319415
  2. study demonstrated that Aiolos silenced the Il2 locus, promoting TH17 differentiation in vitro and in vivo PMID: 22751139
  3. Aiolos knockout mice develop the symptoms of human systemic lupus erythematosus (SLE). This finding implicates Aiolos as a possible candidate gene for SLE. PMID: 12574333
  4. A model is proposed for the control of Bcl-xL's anti-apoptotic role in IL-4-deprived cells via association to the transcription factor Aiolos. PMID: 11714801
  5. Normal Aiolos function is necessary to maintain immune homeostasis and suppress the development of systemic autoimmune disease; Aiolos is implicated as a possible candidate gene for systemic lupus erythematosus. PMID: 12574333
  6. Failure to generate high affinity plasma cells in the bone marrow and to sustain serum antibody titers is apparent after both primary and secondary immunization of Aiolos(-)(/)(-) mice with a range of hapten concentrations. PMID: 14718515
  7. A novel role for OBF-1 and Aiolos in controlling the transcription and nuclear organization of genes involved in pre-BCR function. PMID: 18974788

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Ikaros C2H2-type zinc-finger protein family
Tissue Specificity
Expression is restricted to lymphoid tissues. Expressed at highest levels in spleen and at lower levels in the thymus and bone marrow. First detected in more committed lymphoid progenitors and strongly up-regulated as these differentiate into pre-T and pr
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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