Recombinant Mouse cAMP-dependent protein kinase catalytic subunit PRKX (Prkx)

Code CSB-YP838750MO
MSDS
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Source Yeast
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Code CSB-EP838750MO
MSDS
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Source E.coli
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Code CSB-EP838750MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP838750MO
MSDS
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Source Baculovirus
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Code CSB-MP838750MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Prkx
Uniprot No.
Alternative Names
Prkx; Pkare; cAMP-dependent protein kinase catalytic subunit PRKX; PrKX; Protein kinase X; Protein kinase X-linked; Serine/threonine-protein kinase PRKX; EC 2.7.11.1; PKA-related protein kinase
Species
Mus musculus (Mouse)
Expression Region
1-355
Target Protein Sequence
MEPPAGAAAT VKDPDHDPVK TKVSAPAADP KPRTSSQKAG HSLQDWDTIA TVGTGTFGRV NLVKEKTGRQ YCALKIMSIP DVIRLKQEQH VQNEKAVLKE INHPFLIKLL WTGHDNRFLY MLMEFVPGGE LFTYLRNRGR FSSVASVFYA TEIVCAIEYL HSKEIVYRDL KPENILLDRE GHIKLTDFGF AKKLVDRTWT LCGTPEYLAP EVIQSKGHGR AVDWWALGIL IFEMLSGFPP FFDDNPFGIY QKILACKIDF PRQLDFTSKD LIKKLLVVDR TRRLGNMKNG AEDIKRHRWF RGVEWESVPQ RKLKPPIVPK LSGDGDISNF ETYPESELDK TPSVSDKDLE TFKNF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation.
Gene References into Functions
  1. The interaction of PRKX with Pin-1, Magi-1 and Bag-3 could contribute to the stimulating role of PRKX in angiogenesis. PMID: 21684272
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family, cAMP subfamily
Tissue Specificity
Widely expressed.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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