Name: Recombinant Mycobacterium tuberculosis Hypoxic response protein 1(hrp1)
Brand: CUSABIO
SKU: CSB-EP516950MVZ

Product Details

Purity	Greater than 90% as determined by SDS-PAGE.
Target Names	hrp1
Uniprot No.	P9WJA3
Research Area	others
Alternative Names	hrp1; Rv2626cHypoxic response protein 1; HRP1
Species	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Source	E.coli
Expression Region	1-143aa
Target Protein Sequence	MTTARDIMNAGVTCVGEHETLTAAAQYMREHDIGALPICGDDDRLHGMLTDRDIVIKGLAAGLDPNTATAGELARDSIYYVDANASIQEMLNVMEEHQVRRVPVISEHRLVGIVTEADIARHLPEHAIVQFVKAICSPMALAS Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight	35.5kDa
Protein Length	Full Length
Tag Info	N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form	Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	3-7 business days
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

We have several post-sale questions (CSB-EP516950MVZ):
1. We will not use the whole protein in one shot, and would rather aliquot it. Our question is: which is the best buffer to create a stock solution?
2. What would be the best concentration for this stock solution?
3. Which temperature should it be kept?

Very nice to receive your inquiry.
1. As shown in our Datasheet&COA, the storage buffer of this protein is 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0, 50% glycerol.
You can directly pack it into small specifications according to your needs.
2. The protein concentration of this lot is 0.3mg/ml. We suggest you to make aliquotes directly, and don't suggest to add buffer to dilute the protein before making aliquotes.
The lower the protein concentration, the more unstable.
3. Regarding the storage temperature, if you do not intend to use the protein in a short period, we recommend storing it at -20 ° C to -80 ° C (Be sure to avoid repeated freezing and thawing).

We have two questions:
1. Can aliquots be stored in Matrix tubes, or would you suggest tubes with low binding property for proteins?
2. Can they use HBSS or PBS (ph 7.4) to create an intermediate working solution, prior final dilution in blood or PBMCs?

Thanks for your inquiry.
1. We use Axygen's (an US company) cryotube (99.9% bio-grade polypropylene as raw material, without any metal ions) for storage and transportation.
The customer could make choice according to the tube we used.
2. In theory, the customer can use HBSS or PBS (ph 7.4) to create an intermediate working solution, prior final dilution in blood or PBMCs.
However, we have not done relevant experiment yet, and the customer can have a try firstly.
Please do not to contact us if the customer has further questions. Have a nice day.

Target Background

Function	Unlike some other CBS-domain containing proteins does not seem to bind AMP.
Gene References into Functions	Mycobacterium tuberculosis protein Rv2626c plays a significant role in stimulating macrophages to provoke a pro-inflammatory response and in mycobacterial survival during infection. PMID: 28671529 Rv2626c \appears to modulate macrophage effector functions by eliciting both innate and adaptive immune responses PMID: 20201990
Subcellular Location	Secreted. Note=Not seen to be associated with the cell wall.
Database Links	KEGG: mtu:Rv2626c STRING: 83332.Rv2626c

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Check Code：

Code	CSB-EP516950MVZ
Size	US$2466
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP516950MVZ could indicate that this peptide derived from E.coli-expressed Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) hrp1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP516950MVZ could indicate that this peptide derived from E.coli-expressed Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) hrp1.
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Recombinant Mycobacterium tuberculosis Hypoxic response protein 1(hrp1)

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Target Background

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