Recombinant Neurospora crassa White collar 1 protein(wc-1) ,partial

Code CSB-YP311524NHA
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Source Yeast
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Code CSB-EP311524NHA
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Source E.coli
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Code CSB-EP311524NHA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP311524NHA
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Source Baculovirus
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Code CSB-MP311524NHA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names wc-1
Uniprot No. Q01371
Alternative Names
wc-1; NCU02356; White collar 1 protein; WC1
Species Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

May function as a transcription factor involved in light regulation. Binds and affects blue light regulation of the al-3 gene. Wc-1 and wc-2 proteins interact via homologous PAS domains, bind to promoters of light regulated genes such as frq, and activate transcription.
Gene References into Functions
  1. DNA binding for light induction by the White Collar complex requires only WC-2, whereas DNA binding for circadian functions requires WC-2 . PMID: 26711258
  2. WC-1 stability is reduced in the fastest photocycle mutant even in the presence of clock gene frequency as a second stabilizing factor. PMID: 25978382
  3. The not1 transcript displays a circadian oscillation with a similar phase as frq. Down-regulation of not1 leads to low levels of WC-1 and WC-2 and a delayed circadian phase as a result of increased protein degradation and increased WC activity. PMID: 24030828
  4. A WC-1/NGF-1 complex is preassembled in the dark on light-inducible promoters that, after exposure to light stimulation, leads to histone H3 acetylation and transcriptional activation. PMID: 22875992
  5. light-independent phosphorylation of WC-1 regulates its function in the Neurospora circadian negative feedback loop PMID: 15731099
  6. a common mechanism controls the light-induced transcription of immediate early genes (such as frq, al-3, and vvd) in Neurospora, in which light induces the binding of identical large WC-1/WC-2 complexes to the light response elements in their promoters PMID: 16287715
  7. Light sensitivity and temperature compensation may be conferred by the intact FRQ/WC loop in the WT strain. PMID: 16537415
  8. Data show that WC-1 stability cycles, that WC-1 is stable in the absence of FRQ, and that physical binding between FRQ and WCC is essential for closure of the negative feedback loop. PMID: 18997062

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Subcellular Location Nucleus.
Database Links

KEGG: ncr:NCU02356

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