Recombinant Paracoccus denitrificans Uroporphyrinogen-III C-methyltransferase(nirE)

Product Type Recombinant Protein
Uniprot No. Q51701
Abbreviation nirE
Species Paracoccus denitrificans (strain Pd 1222)
Gene Names nirE
Protein Names Recommended name: Uroporphyrinogen-III C-methyltransferase Short name= Urogen III methylase EC= 2.1.1.107 Alternative name(s): SUMT Uroporphyrinogen III methylase Short name= UROM
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Catalyzes the methylation of both C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin (By similarity). Inactivation of uroporphyrinogen-III methyltransferase results in the loss of nitrite and nitric oxide reductase activities, but not of nitrous oxide reductase activity. Likely involved in heme D1 biosynthesis.
Protein Families Precorrin methyltransferase family
Database Links

KEGG: pde:Pden_2488

STRING: 318586.Pden_2488

Product Details for Different Expression Systems

Product Name Recombinant Paracoccus denitrificans Uroporphyrinogen-III C-methyltransferase(nirE),Yeast
Code CSB-YP684392PYC
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAGKTVTNGA AQGKAARSGA DGAVRGKAGM GRVDLIGAGP GDPELLTLRA LRLLQQADVV VHDRLVSDEV MACIPAHVRR IPVGKAAGFH PVPQEQINAL LVELGLSGLT VARLKGGDPT IFGRGGEEFE AVTRAGIPCD YVPGITAAQG AAVSARFPLT HRGLATGLRH VTGHRARDAA LDLDWASLAD PQTTLAIYMG AANMAEIARE LIRHGMPADL PVLAVSQAST PQEQRLHATL QDIAAALARK PLPAPVLFIV GHVAAMAEDC ALPQELYRPE WRLVAHG
Expression Region 1-287
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Paracoccus denitrificans Uroporphyrinogen-III C-methyltransferase(nirE),E.coli
Code CSB-EP684392PYC
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAGKTVTNGA AQGKAARSGA DGAVRGKAGM GRVDLIGAGP GDPELLTLRA LRLLQQADVV VHDRLVSDEV MACIPAHVRR IPVGKAAGFH PVPQEQINAL LVELGLSGLT VARLKGGDPT IFGRGGEEFE AVTRAGIPCD YVPGITAAQG AAVSARFPLT HRGLATGLRH VTGHRARDAA LDLDWASLAD PQTTLAIYMG AANMAEIARE LIRHGMPADL PVLAVSQAST PQEQRLHATL QDIAAALARK PLPAPVLFIV GHVAAMAEDC ALPQELYRPE WRLVAHG
Expression Region 1-287
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Paracoccus denitrificans Uroporphyrinogen-III C-methyltransferase(nirE),Baculovirus
Code CSB-BP684392PYC
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAGKTVTNGA AQGKAARSGA DGAVRGKAGM GRVDLIGAGP GDPELLTLRA LRLLQQADVV VHDRLVSDEV MACIPAHVRR IPVGKAAGFH PVPQEQINAL LVELGLSGLT VARLKGGDPT IFGRGGEEFE AVTRAGIPCD YVPGITAAQG AAVSARFPLT HRGLATGLRH VTGHRARDAA LDLDWASLAD PQTTLAIYMG AANMAEIARE LIRHGMPADL PVLAVSQAST PQEQRLHATL QDIAAALARK PLPAPVLFIV GHVAAMAEDC ALPQELYRPE WRLVAHG
Expression Region 1-287
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Paracoccus denitrificans Uroporphyrinogen-III C-methyltransferase(nirE),Mammalian cell
Code CSB-MP684392PYC
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAGKTVTNGA AQGKAARSGA DGAVRGKAGM GRVDLIGAGP GDPELLTLRA LRLLQQADVV VHDRLVSDEV MACIPAHVRR IPVGKAAGFH PVPQEQINAL LVELGLSGLT VARLKGGDPT IFGRGGEEFE AVTRAGIPCD YVPGITAAQG AAVSARFPLT HRGLATGLRH VTGHRARDAA LDLDWASLAD PQTTLAIYMG AANMAEIARE LIRHGMPADL PVLAVSQAST PQEQRLHATL QDIAAALARK PLPAPVLFIV GHVAAMAEDC ALPQELYRPE WRLVAHG
Expression Region 1-287
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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