Recombinant Pig Interferon-induced GTP-binding protein Mx1 (MX1), partial

Code CSB-YP015249PI
MSDS
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Source Yeast
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Code CSB-EP015249PI
MSDS
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Source E.coli
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Code CSB-EP015249PI-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015249PI
MSDS
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Source Baculovirus
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Code CSB-MP015249PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MX1
Uniprot No.
Alternative Names
MX1; Interferon-induced GTP-binding protein Mx1; Myxoma resistance protein 1; Myxovirus resistance protein 1
Species
Sus scrofa (Pig)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Interferon-induced dynamin-like GTPase with antiviral activity against influenza A virus, (IAV). Inhibits IAV replication by decreasing or delaying NP synthesis and by blocking endocytic traffic of incoming virus particles.
Gene References into Functions
  1. The deletion/insertion polymorphism in exon 14 of the Mx1 gene, which causes a frame shift, showed no significant association with growth or viremia after PRRSV infection. PMID: 26711047
  2. the SINE insertion polymorphism at site -547 of the MX1 gene promoter region is a potential DNA marker for porcine reproductive and respiratory syndrome resistance PMID: 26095790
  3. Overexpression of porcine Mx1 induce a robust immune response against foot-and-mouth disease virus. PMID: 26047650
  4. Studied the effects of porcine Mx1 protein on FMDV and BVDV replication by measuring viral reverse transcriptase activity at various time intervals. PMID: 25153459
  5. Polymorphisms in Mx1 are significantly associated with immunological traits in Landrace piglets and have potential application value for marker-assisted selection of pig breeding with disease resistance. PMID: 21667240
  6. Porcine Mx1 protein inhibits replication of influenza A virus, and impairs the traffic of the endocytic vesicles to the late endosomes. PMID: 20167191
  7. The 11-bp deletion Mx1 mutant is lacking antiviral activity able to contribute to the interference of influenza virus replication. PMID: 17203407
  8. Data showed that there were abundant MX1 gene polymorphisms in all pig breeds. PMID: 17650486
  9. Sequencing of the 332-bp MX1 promoter region identified 15 substitutions and insertions at three positions in 21 pigs from 15 breeds, in which nine genotypes were classified. PMID: 18254733

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Subcellular Location
Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, perinuclear region.
Protein Families
TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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