Recombinant Pig Matrix metalloproteinase-20 (MMP20)

Code CSB-YP014667PI
MSDS
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Source Yeast
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Code CSB-EP014667PI
MSDS
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Source E.coli
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Code CSB-EP014667PI-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP014667PI
MSDS
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Source Baculovirus
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Code CSB-MP014667PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MMP20
Uniprot No.
Alternative Names
MMP20; Matrix metalloproteinase-20; MMP-20; EC 3.4.24.-; Enamel metalloproteinase; Enamelysin
Species
Sus scrofa (Pig)
Expression Region
108-483
Target Protein Sequence
YRL FPGEPKWKKN TLTYRISKYT PSMTPAEVDK AMEMALQAWS SAVPLSFVRV NAGEADIMIS FETGDHGDSY PFDGPRGTLA HAFAPGEGLG GDTHFDNAEK WTMGMNGFNL FTVAAHEFGH ALGLAHSTDP SALMYPTYKY QNPYGFHLPK DDVKGIQALY GPRKTFTGKP TVPHGPPHNP SLPDICDSSS SFDAVTMLGK ELLFFRDRIF WRRQVHLMSG IRPSTITSSF PQLMSNVDAA YEVADRGMAY FFKGPHYWIT RGFQMQGPPR TIYDFGFPRY VQRIDAAVHL KDTQKTLFFV GDEYYSYDER KRKMDKDYPK NTEEEFSGVN GQIDAAVELN GYIYFFSGPK AYKYDTEKED VVSVLKSNSW IGC
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation.
Gene References into Functions
  1. MMP-20 is the enzyme that processes ameloblastin during the secretory stage of amelogenesis PMID: 20400724
  2. MMP-20 processes dental sialophosphoprotein into smaller subunits in the dentin matrix during odontogenesis PMID: 17046814
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Tissue Specificity
Expressed specifically in the enamel organ.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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