Recombinant Pig Myoblast determination protein 1 (MYOD1)

Code CSB-YP015358PI
MSDS
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Source Yeast
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Code CSB-EP015358PI
MSDS
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Source E.coli
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Code CSB-EP015358PI-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015358PI
MSDS
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Source Baculovirus
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Code CSB-MP015358PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYOD1
Uniprot No.
Alternative Names
MYOD1; MYOD; Myoblast determination protein 1
Species
Sus scrofa (Pig)
Expression Region
1-319
Target Protein Sequence
MELLSPPLRD VDLTGPDGSL CNFATADDFY DDPCFDSPDL RFFEDLDPRL VHVGALLKPE EHSHFPAAAH PAPGAREDEH VRAPSGHHQA GRCLLWACKA CKRKTTNADR RKAATMRERR RLSKVNEAFE TLKRCTSSNP NQRLPKVEIL RNAIRYIEGL QALLRDQDAA PPGAAAAFYA PGPLPPGRGG EHYSGDSDAS SPRSNCSDGM MDYSGPPSGA RRRNCYDGTY YSEAPSEPRP GKNAAVSSLD CLSSIVESIS TESPAAPALL LADTPRESSP GPQEAAAGSE VERGTPTPSP DAAPQCPASA NPNPIYQVL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Gene References into Functions
  1. an Enhancer box and a binding site for a cooperative co-activator of MyoD are present in the promoter region of porcine PPARgamma. PMID: 26944559
  2. Of the eight adult pig tissue types that were tested, the expression of Myf5 and MyoD1 was highest in the muscle tissue. PMID: 25966141
  3. Single nucleotide polymorphisms in the MYOD1 and GDF8 genes are associated with genetic transcription during myogenesis in pigs. PMID: 25377122
  4. Therefore, this study demonstrated that the different regulatory adipogenic roles of MSTN in ADSCs and MSCs act by differentially regulating PPARgamma and MyoD expression. PMID: 25646693
  5. Therefore, the g.489C>T and g.1264C>A SNPs in MYOD1 may be meaningful DNA markers that can be used for improving important porcine economic traits. PMID: 22554470
  6. The total expression profile of MyoD and Pax7 genes suggests that higher muscularity in Pietrain pigs is associated with the presence of a greater number of active satellite stem cells compared to other breeds. PMID: 20888930
  7. SKIP might play a role in the regulation of skeletal muscle development in pigs. PMID: 20336382
  8. Exons and promoters are amplified and sequenced in the 5'UTR region of this gene. PMID: 15306724
  9. Relative MYOD1 expression was not different, but MYOG expression was higher in the (ligated-tube)crowded group embryos. PMID: 18462612
  10. MYOD1 intron 1 DdeI polymorphism was not significantly associated with any meat quality traits tested PMID: 18777094

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Subcellular Location
Nucleus.
Database Links
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