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Recombinant Pig N-acetylgalactosamine-6-sulfatase (GALNS)

Recombinant Pig N-acetylgalactosamine-6-sulfatase (GALNS)

Code	CSB-YP837836PI
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP837836PI
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP837836PI-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP837836PI
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP837836PI
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

GALNS

Uniprot No.

Q8WNQ7

Alternative Names

GALNSN-acetylgalactosamine-6-sulfatase; EC 3.1.6.4; Chondroitinsulfatase; Chondroitinase; Galactose-6-sulfate sulfatase; N-acetylgalactosamine-6-sulfate sulfatase; GalNAc6S sulfatase

Species

Sus scrofa (Pig)

Expression Region

26-522

Target Protein Sequence

APQPP NILLLLMDDM GWGDLGVYGE PSRETPNLDR MAAEGMLFPS FYAANPLCSP SRAALLTGRL PIRTGFYTTN GHARNAYTPQ EIVGGIPDPE HLLPELLKGA GYASKIVGKW HLGHRPQFHP LKHGFDEWFG SPNCHFGPYD NRARPNIPVY RDWEMVGRFY EEFPINLKTG ESNLTQIYLQ EALDFIKRQQ ATHHPFFLYW AIDATHAPVY ASRAFLGTSQ RGRYGDAVRE IDDSVGRIVG LLRDLKIAGN TFVFFTSDNG AALVSAPKQG GSNGPFLCGK QTTFEGGMRE PAIAWWPGHI PAGQVSHQLG SVMDLFTTSL SLAGLEPPSD RAIDGLDLLP AMLQGRLTER PIFYYRGNTL MAATLGQYKA HFWTWTNSWE EFRQGVDFCP GQNVSGVTTH SQEEHTKLPL IFHLGRDPGE RFPLSFASTE YLDALRKITL VVQQHQESLV PGQPQLNVCN PAVMNWAPPG CEKLGKCLTP PESVPEKCSW PH

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

CSB-EP837836PI Recombinant Pig N-acetylgalactosamine-6-sulfatase (GALNS
We are hoping for an active protein, have you received any customer feedback on activity?

Thanks for your inquiry.
Recombinant Pig N-acetylgalactosamine-6-sulfatase(GALNS)
CSB-EP837836PI >> E.coli
Regarding the activity, sorry, we haven't received the feedback from other customers, and we also haven't detected the activity before, it's not 100% guaranteed.
However, as what we provide is the full length of the mature protein and we use the affinity chromatography method to purify in a mild mild condition, theoretically, it should be active. We recommend you to purchase a small size to have a try first.
Below are some literatures for your reference:
http://link.springer.com/article/10.1007%2Fs10295-010-0766-x
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1136156/
http://www.ncbi.nlm.nih.gov/pubmed/17336563