Recombinant Pig Serine/threonine-protein kinase WNK1 (WNK1)

Code CSB-YP740825PI
MSDS
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Source Yeast
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Code CSB-EP740825PI
MSDS
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Source E.coli
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Code CSB-EP740825PI-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP740825PI
MSDS
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Source Baculovirus
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Code CSB-MP740825PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WNK1
Uniprot No.
Alternative Names
WNK1; HSN2; Serine/threonine-protein kinase WNK1; EC 2.7.11.1
Species
Sus scrofa (Pig)
Expression Region
1-411
Target Protein Sequence
QSMAHPRGGT PTYPESQIFF PTIHERPVSF SPPPTCPPKV AISQRRKSTS FLEAQTHHFQ PLLRTVGQNL LPPGGCPTNW TPEAVVMLGT TASRVTGEPC EIQVQPLFEP TQVYGDCRPG LVLPEEAHYF IPQEAVYVAG VHYQTQMAEQ FEGIPYNSPV LSSPMKQIPE QKPVQGGPPS SSVFEFPSGQ AFLVGHLQNL RLDSGLSPGS PLSSISTPIS TDATRLKFHP VFVPHSAPAV LTHNNESRSN CVFEFHVHTP SSSSGEGGVL PQRIYRNRQV AVDLNQEEPP PQSAGLHGRL QPVTEEQHNF QPPELTVSVV EPTGQSWPIG SPEYSSDSSQ ITSSDPSDFQ SPPPTGGTAA PFGSDVSLPF IHLPQTVIQE SPLFFCFPQG TTSPQILSAS FSSGGSALHP Q
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
Subcellular Location
Cytoplasm.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, WNK subfamily
Database Links

UniGene: Ssc.97171

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