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Recombinant Probable ribonuclease VapC4 (vapC4)

Recombinant Probable ribonuclease VapC4 (vapC4)

Code	CSB-YP519214MVZ
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP519214MVZ
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP519214MVZ-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP519214MVZ
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP519214MVZ
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

vapC4

Uniprot No.

O07783

Alternative Names

vapC4; Rv0595cRibonuclease VapC4; RNase VapC4; EC 3.1.-.-; Toxin VapC4; VapC-mt4

Species

Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Expression Region

1-130

Target Protein Sequence

MNVRRALADT SVFIGIEATR FDPDRFAGYE WGVSVVTLGE LRLGVLQASG PEAAARRLST YQLAQRFEPL GIDEAVSEAW ALLVSKLRAA KLRVPINDSW IAATAVAHGI AILTQDNDYA AMPDVEVITI

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Toxic component of a type II toxin-antitoxin (TA) system. Probably exerts its toxic effect by binding to mRNA, inhibiting translation. Binds to, recognizes and cleaves ssRNA at ACGC and AC(A/U)GC sequences, usually between the G and C; cleavage is not very efficient, nor is cleavage required to inhibit protein synthesis. Upon expression in situ, in M.smegmatis or E.coli inhibits cell growth and colony formation; in at least E.coli also causes increased levels of cellular RNA. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB4.

Gene References into Functions

VapC-mt4 alters specific tRNAs to inhibit translation and modulate growth. This stress-linked activity of VapC-mt4 mirrors basic features of eukaryotic tRNases that also generate tRNA halves and inhibit translation in response to stress. PMID: 26158745
The activity of VapC-mt4 is mechanistically distinct from other toxin-toxinS because it appears to primarily inhibit translation through selective, stable binding to RNA. PMID: 22354968

Subcellular Location

Secreted.

Protein Families

PINc/VapC protein family

Database Links

KEGG: mtu:Rv0595c

STRING: 83332.Rv0595c

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