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Recombinant Pseudomonas aeruginosa Chaperone protein DnaK (dnaK), partial

Recombinant Pseudomonas aeruginosa Chaperone protein DnaK (dnaK), partial

Code	CSB-YP875861EZX
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP875861EZX
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP875861EZX-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP875861EZX
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP875861EZX
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

dnaK

Uniprot No.

Q9HV43

Alternative Names

dnaK; PA4761Chaperone protein DnaK; HSP70; Heat shock 70 kDa protein; Heat shock protein 70

Species

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Please provide the following information regarding CSB-EP875861EZX:
1.Sequence
2.Tag type
3.Tag position
4.Pricing, sizes and formats

1)Can you please confirm the bacteria species of the protein?
2)How low is the endotoxin removal if we request endotoxin removal service?
3)Also, we are interested in 0.1mg or 0.05mg of this product (lyophilization).

Thanks for your inquiry.
Recombinant Pseudomonas aeruginosa Chaperone protein DnaK(dnaK) ,partial
CSB-EP875861EZX >> E.coli
Expression Region: 380-637aa; Fragment at the C-terminal.
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

AVLAGDVKDVLLLDVTPLTLGIETLGGVMTGLIEKNTTIPTKKSQVFSTADDNQGAVTIHVLQGERKQAAQNKSLGKFDLADIPPAPRGVPQIEVTFDIDANGILHVSAKDKATGKQQSIVIKASSGLSEDEIQQMVRDAEANAEEDRKFEELAAARNQGDALVHATRKMITEAGDKATAEDKATIEKALGELEAAVKGDDKAEIEAKMNALSQASTPLAQKMYAEQAQQGEDAPQGEQAKAADDVVDAEFEEVKDNK

Reference: https://www.uniprot.org/uniprot/Q9HV43
①The bacteria species of the protein is Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
②Please remark your requirement when you place the order if you require for endotoxin removal, we can only guarantee that the final endotoxin level will be less than <1.0 EU per 1μg of the protein by the LAL method.
③We can provide lyophilization service for free, and probably 6% trehalose will be added as protective agent during the lyophilization,
lead time will be extended for 3 working days.

Target Background

Function

Acts as a chaperone.

Gene References into Functions

Inactivation of dnaK caused significant repression of bacterial penetration through Caco-2 cell monolayers, with decreased swimming, swarming and twitching motilities. PMID: 28758636

Protein Families

Heat shock protein 70 family

Database Links

KEGG: pae:PA4761

STRING: 208964.PA4761

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