Recombinant Pseudomonas aeruginosa Dihydrolipoyl dehydrogenase 3 (lpd3)

Code CSB-YP862214EZX
MSDS
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Source Yeast
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Code CSB-EP862214EZX
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Source E.coli
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Code CSB-EP862214EZX-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862214EZX
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Source Baculovirus
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Code CSB-MP862214EZX
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
lpd3
Uniprot No.
Alternative Names
lpd3; PA4829Dihydrolipoyl dehydrogenase 3; EC 1.8.1.4; Dihydrolipoamide dehydrogenase 3; LPD-3
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Expression Region
1-467
Target Protein Sequence
MMESYDVIVI GAGPGGYNAA IRAGQLGLKV ACVEGRETLG GTCLNVGCMP SKALLHASEL YAAASGGEFA RLGIRVSPEL DLAQMMKQKD ESVAALTRGV EFLFRKHKVQ WIKGWARLQG EGRVGVALAD GGHAQLEARD IVIATGSEPA PLPGVPVDNQ RILDSTGALE LVEVPRHLVV IGAGVIGLEL GSVWRRLGAQ VTVLEYLERI CPGLDGETAR TLQRALTRQG MRFRLGTRVV AARSGEQGVE LDLQPAAGGA TESLQADYVL VAIGRRPYTE GLGLETVGLA SDRRGMLENQ GQRSAAPGVW VIGDVTSGPM LAHKAEEEAI VCIERIAGHA AEMNAEVIPS VIYTQPEVAS VGLGEEQLQA ARREYKVGRF PFSANSRAKI NHESEGFIKI LSDARSDQVL GVHMIGPGVS EMIGEACVAM EFSASAEDLA LTCHPHPTRS EALRQAAMDV HGRAMQN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing.
Gene References into Functions
  1. In contrast to the notion that phenazines support intracellular redox homeostasis by oxidizing NADH, our work suggests that phenazines may substitute for NAD(+) in LpdG and other enzymes, achieving the same end by a different mechanism PMID: 28174304
  2. Lpd of P. aeruginosa is a surface exposed moonlighting protein that binds two human terminal pathway inhibitors, vitronectin and clusterin PMID: 26368530
Subcellular Location
Cytoplasm.
Protein Families
Class-I pyridine nucleotide-disulfide oxidoreductase family
Database Links

KEGG: pae:PA4829

STRING: 208964.PA4829

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