Recombinant Pseudomonas syringae pv. tomato Alginate biosynthesis protein AlgJ (algJ)

Code CSB-YP801531FGP
MSDS
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Source Yeast
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Code CSB-EP801531FGP
MSDS
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Source E.coli
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Code CSB-EP801531FGP-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP801531FGP
MSDS
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Source Baculovirus
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Code CSB-MP801531FGP
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
algJ
Uniprot No.
Alternative Names
algJ; PSPTO_1234Probable alginate O-acetylase AlgJ; EC 2.3.1.-; Alginate biosynthesis protein AlgJ
Species
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Expression Region
36-391
Target Protein Sequence
ETTVL NGKWTKAAET HYDDEFPIKR LGTNLWAALD FKLFNEGRPG VVLGKDQWLY TDEEFDAVAN GEQNEADNLA LIQGVRDALE KQGSKLVLAI VPAKTRLYPE HIGDNKPASL HADLYQQFHA QVAKAGIFAP DLLAPLQAAK QQGQVFLRTD THWTPMGAEV AAQQLGAAIA QKAPLEGEPE QFVTQAIETA PYKGDLTTFL PLDPLFSNLL PKPDELQKRS TDPVAGEAAG GDALFADSDV AVGLVGTSYS ANPNWNFVGA LKQALRSDVV NYAEDGHGPI LPMLKYLQTD AFKNTPPQVV IWEFPERYLP AHNDLGEFDP KWIAELKKSR DTQENVALNA KPSESPNRAQ N
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Together with AlgI and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization-transport complex and adds acetyl groups at the O-2 and O-3 positions of mannuronate residues. Acetylation of alginate is important for the architecture of biofilms and increases the ability of alginate to act as a defense barrier.
Subcellular Location
Cell inner membrane; Peripheral membrane protein; Periplasmic side. Periplasm.
Protein Families
AlgJ family
Database Links
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