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Recombinant Pseudomonas syringae pv. tomato Effector protein HopAB2 (hopAB2), partial

Recombinant Pseudomonas syringae pv. tomato Effector protein HopAB2 (hopAB2), partial

Code	CSB-YP854628FGP
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP854628FGP
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP854628FGP-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP854628FGP
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP854628FGP
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

hopAB2

Uniprot No.

Q8RSY1

Alternative Names

hopAB2; avrPtoB; PSPTO_3087; Effector protein HopAB2; Avirulence protein AvrPtoB) [Includes: E3 ubiquitin-protein ligase; EC 2.3.2.-; E3 ubiquitin-protein transferase)]

Species

Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Effector protein involved in gene-for-gene resistance in tomato plants. It is recognized by the host Pto resistance protein and elicits Pto and Prf-dependent hypersensitive response (HR) and programmed cell death (PCD), resulting in host immunity. In susceptible plants, acts as a virulence factor by suppressing PCD and HR-based plant immunity. This function requires its E3 ubiquitin ligase activity probably by recruiting E2 enzymes and transferring ubiquitin molecules to cellular proteins involved in regulation of PCD and targeting them for degradation. Also, induces expression of host genes involved in ethylene biosynthesis and signaling, in particular ACO1 and ACO2, encoding the ethylene-forming enzyme ACC oxidase.

Gene References into Functions

This work uncovers a strategy in which Pseudomonas syringae AvrPtoB targets Arabidopsis NPR1 and represses NPR1-dependent salicylic acid signaling, thereby subverting plant innate immunity. PMID: 29174403
AvrPto and AvrPtoB have phenotypically redundant avirulence activity on Pto-expressing tomato and additive virulence activities on susceptible tomato plants. PMID: 15672817
we propose that both domains of Pseudomonas syringae pv tomato DC3000 AvrPtoB act together to support the virulence of PtoDC3000 in Arabidopsis through their ability to eliminate FLS2 from the cell periphery. PMID: 19062288

Subcellular Location

Secreted. Note=Secreted via type III secretion system (TTSS). Localized to the plant cell cytoplasm.

Protein Families

HopAB family

Database Links

KEGG: pst:PSPTO_3087

STRING: 223283.PSPTO_3087

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