Recombinant Pseudomonas syringae pv. tomato Harpin hrpZ (hrpZ)

Code CSB-YP768495FGP
MSDS
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Source Yeast
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Code CSB-EP768495FGP
MSDS
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Source E.coli
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Code CSB-EP768495FGP-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP768495FGP
MSDS
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Source Baculovirus
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Code CSB-MP768495FGP
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
hrpZ
Uniprot No.
Alternative Names
hrpZ; PSPTO_1382Harpin HrpZ; Harpin-Pst; HrpZ-Pst protein
Species
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Expression Region
1-370
Target Protein Sequence
MQALNSISSL QTSASLFPVS LNSDVSANTS TSSKELKAVI DQLVQALTQS GQLDETSPLG KMLAKAMAAD GKSANSIDDI TASLDKLIHE KLGDNFGASA GIGAGGGGGG IGGAGSGSGV GGGLSSDAGA GQSDLMSQVL NGLGKAVLDD LLTPSGEGGT TFSSDDMPTL EKVAQFMDDN KAQFPTRDGG SWMNELKEDN GLDAQETAQF RSALDVIGQQ LGQQQGDASG VTSGGGLGSP VSDSSLGNPA IDANTGPAAN GNASVDVGQL IGQLIDRGLQ SVSSGGGLGT PVDNSTQPTG GTPAANPTGN VSNQDLGQLL SGLLQRGLEA TLQDAGNTGA DLQSSAAQVA AQLINALLQG TNNQTNQAVA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Harpins are proteins able to elicit hypersensitive response (HR) in non-host plants and are required for pathogenicity in host plants. HrpZ forms ion-conducting pores permeable for cations. Such pore-forming activity may allow nutrient release and/or delivery of virulence factors during bacterial colonization of host plants.
Gene References into Functions
  1. Our results suggest that over-expression of the hrpZm gene significantly enhances Phytophthora root and stem rot tolerance in soybean by eliciting resistance responses mediated by multiple defense signaling pathways, thus providing an alternative approach for development of soybean varieties with improved tolerance against the soil-borne pathogen Phytophthora root and stem rot . PMID: 29728957
Subcellular Location
Secreted. Host cell membrane. Note=Secreted via type III secretion system (TTSS), delivered into the host intercellular space. HrpZ travels through the hrp pilus and it is secreted only from the pilus tip.
Protein Families
Harpin HrpZ family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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