Recombinant Rat 3-mercaptopyruvate sulfurtransferase (Mpst)

Code CSB-YP014770RA
MSDS
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Source Yeast
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Code CSB-EP014770RA
MSDS
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Source E.coli
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Code CSB-EP014770RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP014770RA
MSDS
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Source Baculovirus
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Code CSB-MP014770RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mpst
Uniprot No.
Alternative Names
Mpst; 3-mercaptopyruvate sulfurtransferase; MST; EC 2.8.1.2
Species
Rattus norvegicus (Rat)
Expression Region
2-297
Target Protein Sequence
AAPQLFRAL VSAQWVAEAL KSPRASQPLK LLDASWYLPK LGRDARREFE ERHIPGAAFF DIDRCSDHTS PYDHMLPSAT HFADYAGSLG VSAATHVVIY DGSDQGLYSA PRVWWMFRAF GHHSVSLLDG GFRYWLSQNL PISSGKSPSE PAEFCAQLDP SFIKTHEDIL ENLDARRFQV VDARAAGRFQ GTQPEPRDGI EPGHIPGSVN IPFTEFLTSE GLEKSPEEIQ RLFQEKKVDL SKPLVATCGS GVTACHVVLG AFLCGKPDVP VYDGSWVEWY MRAQPEHVIS QGRGKTL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.
Gene References into Functions
  1. Presented is alternative pathway of production of hydrogen sulfide and/or polysulfides from (poly)sulfurated catalytic-site cysteine of reaction intermediates of MST via thioredoxin reduction. PMID: 29331374
  2. observations indicate that 3MST is probably not responsible for the increased production of H2S following permanent middle cerebral artery occlusion. PMID: 23805308
  3. 3-Mercaptopyruvate sulphurtransferase and not cystathionine gamma-lyase is the primary regulator of coronary artery hydrogen sulfide production and function. PMID: 26519030
  4. Hydrogen sulfide (H2 S) is synthesized in peripheral neurons through two mitochondrial enzymes, cysteine aminotransferase (CAT) and mercaptopyruvate sulfurtransferase (MPST). PMID: 24611772
  5. 3MST exists in the neurons of medullary respiratory nuclei and its expression can be up-regulated by chronic intermittent hypoxia in adult rat PMID: 24051007
  6. SiRNA silencing of 3-MPST reduced basal bioenergetic parameters and prevented the stimulating effect of 3-MP on mitochondrial bioenergetics. PMID: 23104984
  7. Thus, sulfur oxides are suggested to release in the redox cycle of persulfide of mercaptopyruvate sulfurtransferase. PMID: 22149235
  8. 3-mercaptopyruvate sulfurtransferase (3MST) and cysteine aminotransferase (CAT) are localized to vascular endothelium in the thoracic aorta and produce hydrogen sulfide. PMID: 19605461
  9. the redox state regulates MST activity at the enzymatic level, and MST controls redox to maintain cellular redox homeostasis PMID: 16107337

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Subcellular Location
Cytoplasm. Mitochondrion. Cell junction, synapse, synaptosome.
Tissue Specificity
Expressed in liver, heart, kidney and brain. Localizes to tubular epithelium in the kidney, pericentral hepatocytes in the liver, cardiac cells in the heart and neuroglial cells in the brain. Also expressed in vascular endothelium of the thoracic aorta. W
Database Links
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