Recombinant Rat APOBEC1 complementation factor (A1cf)

Code CSB-YP001002RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP001002RA
MSDS
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Source E.coli
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Code CSB-EP001002RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001002RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP001002RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
A1cf
Uniprot No.
Alternative Names
A1cf; Acf; AspAPOBEC1 complementation factor; APOBEC1-stimulating protein
Species
Rattus norvegicus (Rat)
Expression Region
1-594
Target Protein Sequence
MESNHKSGDG LSGTQKEAAL RALVQRTGYS LVQENGQRKY GGPPPGWDTT PPERGCEIFI GKLPRDLFED ELIPLCEKIG KIYEMRMMMD FNGNNRGYAF VTFSNKQEAK NAIKQLNNYE IRNGRLLGVC ASVDNCRLFV GGIPKTKKRE EILSEMKKVT EGVVDVIVYP SAADKTKNRG FAFVEYESHR AAAMARRRLL PGRIQLWGHP IAVDWAEPEV EVDEDTMSSV KILYVRNLML STSEEMIEKE FNSIKPGAVE RVKKIRDYAF VHFSNREDAV EAMKALNGKV LDGSPIEVTL AKPVDKDSYV RYTRGTGGRN TMLQEYTYPL SHVYDPTTTY LGAPVFYTPQ AYAAIPSLHF PATKGHLSNR ALIRTPSVRE IYMNVPVGAA GVRGLGGRGY LAYTGLGRGY QVKGDKRQDK LYDLLPGMEL TPMNTISLKP QGVKLAPQIL EEICQKNNWG QPVYQLHSAI GQDQRQLFLY KVTIPALASQ NPAIHPFTPP KLSAYVDEAK RYAAEHTLQT LGIPTEGGDA GTTAPTATSA TVFPGYAVPS ATAPVSTAQL KQAVTLGQDL AAYTTYEVYP TFAVTTRGDG YGTF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also seems to protect the edited APOB mRNA from nonsense-mediated decay.
Gene References into Functions
  1. N-terminal 320 amino acids of ACF retained RNA-binding selectivity and supported complementation of editing activity and in addition demonstrate a novel RNA-bridged multimerization of ACF. PMID: 20541536
  2. promoter demonstrates a progressive loss of CpG dinudleotide methylation towards the core promoter region that is entirely unmethylated PMID: 12359328
  3. role in in vitro deamination of cytosine to uracil in single-stranded DNA PMID: 12697753
  4. Splice variants of this protein are associated with different capacities to support apoB mrna editing. PMID: 14570923
  5. ACF plays a crucial role, which is independent of apobec-1 expression, in cell survival, particularly during early embryonic development PMID: 16055734
  6. phosphorylation of ACF by protein kinase C may be a key regulatory mechanism of apoB mRNA editing in rat hepatocytes PMID: 17229474

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Subcellular Location
Nucleus. Endoplasmic reticulum. Cytoplasm. Note=Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum. Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1.
Tissue Specificity
Isoforms 1 and 2 are widely expressed while isoforms 3 and 4 are restricted to liver and small intestine.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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