Product Details

Purity

>85% (SDS-PAGE)

Target Names

Fto

Uniprot No.

Q2A121

Alternative Names

FtoAlpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; U6 small nuclear RNA; 2'-O-methyladenosine-N(6)-)-demethylase FTO; EC 1.14.11.-; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO; EC 1.14.11.-; mRNA; 2'-O-methyladenosine-N(6)-)-demethylase FTO; m6A(m)-demethylase FTO; EC 1.14.11.-; mRNA N(6)-methyladenosine demethylase FTO; EC 1.14.11.53; tRNA N1-methyl adenine demethylase FTO; EC 1.14.11.-

Species

Rattus norvegicus (Rat)

Expression Region

1-502

Target Protein Sequence

MKRVQTAEER EREAKKLRLL EELEDTWLPY LTPKDDEFYQ QWQLKYPKLV FREAGSIPEE LHKEVPEAFL TLHKHGCLFR DLVRIQGKDV LTPVSRILIG DPGCTYKYLN TRLFTVPWPV KGCTINYTEA EIAAACQTFL KLNDYLQVET IQALEELAIK EKANEDAVPL CMAEFPRAGV GPSCDDEVDL KSRAAYNVTL LNFMDPQKMP YLKEEPYFGM GKMAVSWHHD ENLVDRSAVA VYSYSCEGSE DESDDESSFE GRDPDTWHVG FKISWDIETP GLTIPLHQGD CYFMLDDLNA THQHCVLAGS QPRFSSTHRV AECSTGTLDY ILQRCQLALQ NVLNDSDNGD VSLKSFEPAV LKQGEEIHNE VEFEWLRQFW FQGNRYKICT DWWCEPMTQL EGLWKKMESV TNAVLREVKR EGLSVEQRSE ILSAVLIPLT MRQNLRKEWH ARCQARVVRT LPAQQKPDCR PYWEKDDPSM PLPFDLTDVV SEIRSQLLEA RS

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA. Demethylation of m6A(m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping. Also acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Also able to repair alkylated DNA and RNA by oxidative demethylation: demethylates single-stranded RNA containing 3-methyluracil, single-stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Ability to repair alkylated DNA and RNA is however unsure in vivo. Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation. Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells. Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs.

Gene References into Functions

Study revealed that diet was an important factor modulating transcription of Fto and Irx3, but its effect is time-dependent. In contrast, the DNA methylation profiles of Fto and Irx3 were not altered by nutrition, which may indicate that the feeding type, when applied postnatally, did not affect DNA methylation of these genes. PMID: 28179100
demonstrates leptin-induced FTO upregulation in cardiomyocytes via JAK2/STAT3- dependent CUX1 upregulation and suggests an FTO regulatory function of leptin PMID: 24019958
Both Fto mRNA and catechol-O-methyltransferaze mRNA are upregulated in an identical time-dependent manner in fasting animals. PMID: 23671692
increased levels in the liver are involved in oxidative stress and lipid deposition, which characterize nonalcoholic fatty liver disease PMID: 23329013
Maternal obesity significantly impacted FTO expression in both hypothalamus and liver at weaning. PMID: 21980407
Results provide the first evidence that the LepRb-STAT3 signaling pathway is involved in the brain FTO downregulation during ER. PMID: 21267512
this providse the first indication that selective alteration of FTO levels in the hypothalamus can influence food intake, a finding consistent with the reported effects of FTO alleles on appetite and food intake in man PMID: 20098739
FTO could participate in the central control of energy homeostasis. PMID: 18218688

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Subcellular Location

Nucleus. Nucleus speckle. Cytoplasm.

Protein Families

Fto family

Tissue Specificity

Ubiquitous. Highly expressed in teeth and weakly in bone.

Database Links

KEGG: rno:291905

STRING: 10116.ENSRNOP00000015718

UniGene: Rn.229320

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Recombinant Rat Alpha-ketoglutarate-dependent dioxygenase FTO (Fto)

Product Details

Customer Reviews and Q&A

Target Background

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