Recombinant Rat BRCA1-associated RING domain protein 1 (Bard1)

Code CSB-YP889904RA
MSDS
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Source Yeast
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Code CSB-EP889904RA
MSDS
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Source E.coli
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Code CSB-EP889904RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP889904RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP889904RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Bard1
Uniprot No.
Alternative Names
Bard1BRCA1-associated RING domain protein 1; BARD-1; EC 2.3.2.27; RING-type E3 ubiquitin transferase BARD1
Species
Rattus norvegicus (Rat)
Expression Region
1-768
Target Protein Sequence
MPRRPPRVCS GNKPPPVPAM EPATDGLWAH SRAALARLEK LLRCSRCANI LREPVCLGGC EHIFCSGCIS DCVGSGCPVC HTPAWILDLK INRQLDSMIQ LYSKLQNLLH DNKGSDSKDD TSRASLFGDA ERKKNSVKMW FSPRSKKIRC VVNKVSVQTQ PQKAKDDKAQ EASVFEFVSA TPPVVVSTRA KTASRTSAKK HPKKSVAKIN REGNFRPETR DSRFDSKEKL KEEKVVSFSQ TLVMENSRVN GEIDLLASGS VVESVFSGSF AEVSLPLAEH IVSPDTVSKS EEAPEKKVCV EDRCPVGSDG NPKGCHRPPT STSKKCGSNV PSASGEIREP TLLAENVVLV DCSSLPSGRL QVDVTLRRQS NASDDSLSLS PGTPPSLLNN STHRQMMSKP STVKLSSGIP ARKRNHRGET LLHIASIKGD ISSVEYLLQN GNDPNVKDHA GWTPLHEACS HGHLKIVELL LQHNALVNTT GYHNDSPLHD AAKNGHIDIV KVLLSHGASR NAVNIFGERP VDYTDAENIR SLLLLPEKTD SFSTSQCSVQ VNTGQRKSGP LVLIGSGLSS QQQKLLSKLE TVLKAKKCAE FDNTVTHVIV PDEEAQSTLK CMLGILNGCW VLKFDWVKAC LDSQEREQEE KYEVPGGPQR SRLNREQLLP KLFDGCYFFL GGNFKHHPKE DLLKLIAAAG GRILSRKPKP DSDVTQTINT VAYHAKPDSD QRFCTQYIVY EDLFNCHPER VRQGKVWMAP STWLISCVMA FELLPLDS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
Gene References into Functions
  1. Assignment of the BRCA1-associated RING domain gene (Bard1) to rat chromosome 9q34 by in situ hybridization and radiation hybrid mapping PMID: 11856892
  2. Full-length BARD1 might be involved in apoptotic control in spermatogonia and primary spermatocytes. Switch to BRCA1-independent BARD1beta might be necessary to induce apoptosis in BRCA1-expressing meiotic spermatocytes and early round spermatids. PMID: 15240424
Subcellular Location
Nucleus. Cytoplasm.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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