Recombinant Rat Coronin-1A (Coro1a)

Code CSB-YP838714RA
MSDS
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Source Yeast
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Code CSB-EP838714RA
MSDS
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Source E.coli
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Code CSB-EP838714RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP838714RA
MSDS
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Source Baculovirus
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Code CSB-MP838714RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Coro1a
Uniprot No.
Alternative Names
Coro1a; Coro1Coronin-1A; Coronin-like protein A; Clipin-A; Tryptophan aspartate-containing coat protein; TACO
Species
Rattus norvegicus (Rat)
Expression Region
2-461
Target Protein Sequence
SRQVVRSSK FRHVFGQPAK ADQCYEDVRV SQTTWDSGFC AVNPKFMALI CEASGGGAFL VLPLGKTGRV DKNVPLVCGH TAPVLDIAWC PHNDNVIASG SEDCTVMVWE IPDGGLVLPL REPVVTLEGH TKRVGIVAWH PTAQNVLLSA GCDNVILVWD VGTGAAVLTL GPDVHPDTIY SVDWSRDGAL ICTSCRDKRV RVIEPRKGTV VAEKERPHEG TRPVHAVFVS EGKILTTGFS RMSERQVALW DTKHLEEPLS LQELDTSSGV LLPFFDPDTN IVYLCGKGDS SIRYFEITSE APFLHYLSMF SSKESQRGMG YMPKRGLEVN KCEIARFYKL HERKCEPIAM TVPRKSDLFQ EDLYPPTAGP DPALTAEEWL SGRDAGPLLI SLKDGYVPPK SRELRVNRGL DSARRRATPE PSSTLSSDTV SRLEEDVRNL NAIVQKLQER LDRLEETVQA K
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion.
Gene References into Functions
  1. Formation of filopodia by membrane glycoprotein M6a (Gpm6a) requires actin regulator coronin-1a (Coro1a), known to regulate plasma membrane localization and activation of Rac1 and its downstream effector Pak1. PMID: 26809475
  2. Coronin 1A has an inhibitory effect on neurite outgrowth in vitro. PMID: 25179994
  3. Coronin 1A preferentially binds to PI(4,5)P(2)-containing liposomes and PI(4,5)P(2) antagonizes the ability of Coronin 1A to disassemble actin filament branches, indicating spatiotemporal regulation of Coronin 1A via direct interaction withmembrane lipid PMID: 20032464
Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Cytoplasmic vesicle, phagosome membrane.
Protein Families
WD repeat coronin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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