Recombinant Rat Cyclic AMP-dependent transcription factor ATF-3(Atf3)

Code CSB-YP002271RA
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Source Yeast
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Code CSB-EP002271RA
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Source E.coli
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Code CSB-EP002271RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002271RA
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Source Baculovirus
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Code CSB-MP002271RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Atf3
Uniprot No. P29596
Alternative Names Atf3; Lrf1; Cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; Activating transcription factor 3; Liver regeneration factor 1; LRF-1
Species Rattus norvegicus (Rat)
Expression Region 1-181
Target Protein Sequence MMLQHPGQVS ASEVSATAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS ALESVTINNR PLEMSVTKSE VAPEEDERKR RRRERNKIAA AKCRNKKKEK TECLQKESEK LESVNAELKA QIEELKNEKQ HLIYMLNLHR PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ S
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
In solution, it binds the cAMP response element (CRE) (consensus
Gene References into Functions
  1. Data suggest that heat stress enhances ovarian levels of Hsp70 and affects Erk1/2 and Atf3 phosphorylation levels, but not c-Akt activation/phosphorylation, in pseudo-pregnant rats during luteal regression induced by cloprostenol, a prostaglandin F2alpha analog. (Hsp70 = heat shock 70 kDa protein; Erk = extracellular signal-regulated kinase; Atf3 = activating transcription factor-3; c-Akt = proto-oncogene protein c-Akt) PMID: 27169499
  2. ATF3 was highly expressed in the livers of ZDF rats. Insulin resistance and hepatic steatosis were associated with increased ATF3 expression and decreased fatty acid oxidation via mitochondrial dysfunction and were attenuated by in vivo ATF3 silencing. Knockdown of ATF3 also ameliorated glucose intolerance, impaired insulin action, and inflammatory responses in ZDF rats. PMID: 28365312
  3. The cellular injury/stress response induced in sensory neurons by tissue damage and indicated by ATF3 expression is positioned to contribute to pain which can occur after tissue damage. PMID: 27264359
  4. Heme oxygenase-1/signal transducer and activator of transcription 3 signaling plays a critical role in propofol-mediated amelioration of hyperglycemia-induced cardiomyocyte hypertrophy and apoptosis. PMID: 24810525
  5. satellite glial cells (SGCs) play an important role in articular inflammation, with 7 days of disease being a critical time-point in the arthritis model, and suggest that ATF3 might be involved in SGCs' reactive changes such as activation. PMID: 25247596
  6. this is the first study to provide evidence for temporal relationships between MAPK activation and ATF3 expression during PGF-induced luteal regression in the rat. PMID: 25614048
  7. our data demonstrate that a cell-autonomous program consisting of a stress-induced Atf3-gelsolin cascade affects the change in dendritic spine morphology in models of tuberous sclerosis complex PMID: 26224859
  8. ATF3 activation caused induction of modulators of the inflammatory response NF-kappaB and Nkx-2.5, as well as attenuation of pro-fibrotic and pro-inflammatory proteins IL-6 and PAI-1. PMID: 25136830
  9. ATF3 is a novel nuclear marker for migrating ependymal stem cells in the spinal cord. PMID: 24801224
  10. Atf3-silencing reversed ethanol-mediated Gck down-regulation and beta-cell dysfunction, followed by the amelioration of impaired glucose tolerance and insulin resistance. PMID: 25074928
  11. ATF3 may play as a counteracting regulator of AMPK and thus promote beta-cell dysfunction and the development of type-2 diabetes and could be a potential therapeutic target in treating type-2 diabetes. PMID: 23916985
  12. Epithelium-derived exosomal ATF3 RNA attenuates ischemia-reperfusion induced kidney injury by inhibiting MCP-1 gene transcription. PMID: 24420912
  13. Expression of ATF3 in uninjured dorsal root ganglion neurons may contribute to pain following brachial plexus avulsion injury. PMID: 23471575
  14. Penetrating ballistic-like brain injury alone significantly increased ATF3 in microglia/macrophages, NKCs, astrocytes, and neurons. NNZ-2566 further increased this. PBBI decreased Atf3 levels in T cells but this was reversed by NNZ-2566. PMID: 23765588
  15. Psychological stress following restraint induces the endoplasmic reticulum stress gene, ATF3. PMID: 23644055
  16. Diabetic BB rats display an increased activation of ATF 3 and also a rise in apoptotic caspase 3 expression in Schwann cells. PMID: 22446192
  17. Loss of Atf3 causes abnormal cardiomyocyte growth, presumably resulting from the dysregulation of target genes. PMID: 22390138
  18. ATF3 plays an important role in caspase-dependent neuronal apoptotic signal transduction pathways caused by focal cerebral ischemia and reperfusion injury. PMID: 21616101
  19. Activating transcription factor 3 expression is definitely involved in monoarthritic rat model of chronic inflammatory joint pain, marking injured neurons. PMID: 21912089
  20. Exposure to 95% oxygen enhanced ATF4 immunoreactivity and nuclear protein content in whole lung extracts, followed by a 2- and 5-fold increase in ATF3 protein and mRNA expression, respectively. PMID: 21984568
  21. Skin incision without nerve damage induces expression of ATF3 mRNA and protein and alters expression of other regeneration-associated gene transcripts. PMID: 20627820
  22. expression of ATF3 was upregulated in smooth muscle cells in the injured mouse femoral arteries compared with the uninjured control group PMID: 21280179
  23. ATF-3 is co-localized with other cell-specific markers in the spinal cord, dorsal root ganglia (DRG), and in dorsal and ventral roots of the G93A SOD1 rat model of ALS. PMID: 20470869
  24. ATF3 as an important early transcriptional regulator of cyclic-stretch-induced responses PMID: 20413626
  25. findings indicate that upregulation of ATF3 gene expression is involved in regulating glomerular mesangial cell apoptosis induced by sublytic C5b-9 complexes. PMID: 20140008
  26. The neurons that fail to regenerate axons in peripheral injury, there is a much reduced expression of ATF3 transcripts in comparison to the expression in regenerating neurons. PMID: 19913522
  27. a neuronal injury marker PMID: 11936907
  28. Overexpression of ATF3 inhibits mitogen-activated kinase kinase kinase 1 (MEKK1)-c-Jun N-Terminal Kinase (JNK)-induced apoptosis and activates AKT inducing HSP27 expression and neurite elongation in PC12 cells and superior nerve ganglion neurons PMID: 12832543
  29. Trigeminal ganglion neurons in culture expressed ATF3 compared to undetectable expression in native ganglia. In addition, NGF was unable to rescue this injured phenotype. Thus, sensory neurons in culture represent a potential model of injured neurons. PMID: 14559366
  30. We show that expression of ATF3 with c-Jun significantly enhances c-Jun-mediated neurite sprouting. Our results suggest that a program of axonal regeneration is initiated when both c-Jun and ATF3 are upregulated in neurons in response to axotomy. PMID: 14667575
  31. Overexpression of exogenous ATF-3 was found to protect vulnerable neurons from intra-hippocampal injection of kainic acid suggesting an anti-apoptotic role for ATF-3 in the brain PMID: 15135228
  32. activating transcription factor 3 has a role in adenylyl cyclase VI regulation of phospholamban expression in cardiac myocytes PMID: 15231818
  33. The results suggest that retrograde axonal transport of JNK signaling components contributes to the injury induced c-Jun phosphorylation and ATF3 induction. PMID: 15911351
  34. ATF3 plays a role in mediating the cell proliferation function of c-Myc PMID: 15990869
  35. ATF3deltaZip2 counteracts anti-apoptotic activity of NF-kappaB. PMID: 16291753
  36. ATF-3 may contribute, therefore, to neurite outgrowth by orchestrating the gene expression responses in injured neurons. PMID: 16713293
  37. Collectively, these data suggest that this putative model of OA is associated with an early phase neuropathy in the L5 innervation territory of the knee. PMID: 17276007
  38. MAPK signaling and ATF3 action are essential for full SgII promoter activation by GnRHa through a canonical CRE PMID: 17962349
  39. ATF3 overexpression in nucleus accumbens decreases emotional reactivity and increases depression-like behavior. PMID: 18305237
  40. Data show that delayed nerve repair impairs nerve regeneration and length of axonal outgrowth correlates to ATF3 expression in both neurons and Schwann cells. PMID: 18801180
  41. regulation of three immediate early genes which are responsive to endothelin-1 in cardiac myocytes: Atf3, Klf2 and IL6. PMID: 19192484
  42. ATF3 plays a critical role in toxicity induced by mutant N-terminal huntingtin fragment with expanded polyglutamine PMID: 19559011

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Subcellular Location Nucleus
Protein Families BZIP family, ATF subfamily
Tissue Specificity In tissues containing skeletal muscle or smooth muscle, and at low levels in some tumor cells.
Database Links

KEGG: rno:25389

STRING: 10116.ENSRNOP00000005085

UniGene: Rn.9664

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