Recombinant Rat FMRFamide-related peptides (Npvf)

Code CSB-YP861710RA
MSDS
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Source Yeast
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Code CSB-EP861710RA
MSDS
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Source E.coli
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Code CSB-EP861710RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861710RA
MSDS
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Source Baculovirus
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Code CSB-MP861710RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Npvf
Uniprot No.
Alternative Names
Npvf; Rfrp; Pro-FMRFamide-related neuropeptide VF; FMRFamide-related peptides) [Cleaved into: Neuropeptide NPSF; Neuropeptide RFRP-1; Neuropeptide NPVF; Neuropeptide RFRP-2)]
Species
Rattus norvegicus (Rat)
Expression Region
58-94
Target Protein Sequence
SVT FQELKDWGAK KDIKMSPAPA NKVPHSAANL PLRF
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Neuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP. Neuropeptide NPVF blocks morphine-induced analgesia.
Gene References into Functions
  1. study concluded that RFamide-related peptide- (3RFRP-3) plays a regulatory role in male rat reproduction PMID: 29913425
  2. Rfrp (GnIH)-expressing neurons serve as potential upstream mediators of stress-induced effects through Gpr147 (GnIH-R)-dependent mechanisms. PMID: 24412804
  3. Hypothalamic expression of Rfrp and Rfrp1r receptor in rats peaks during the pubertal period in males (postnatal day (PND)-35 to PND-42) but not in females PMID: 24823392
  4. Both acute and chronic immobilization stress lead to an up-regulation of RFRP expression in the dorsomedial hypothalamus (DMH) of adult male rats. PMID: 19541621
  5. The hypothalamic RFRP system changes during development. Ovarian sex steroid estradiol-17beta (E2) may stimulate mRNA expression of RFRP in the prepubertal period when the basal E2 concentration is low. PMID: 22064075
  6. RFRP-3 inhibits secretion of LH and FSH inmammals. PMID: 20424142
  7. Developmental and steroidogenic effects on the gene expression of RFamide related peptides and their receptor in the rat brain and pituitary gland. PMID: 20136694
  8. These results indicate that RFRP-3 has similar effects on LH as observed with GnIH in avian species; in the rat RFRP-3 has additional roles in regulating feeding and growth. PMID: 17113584
  9. Endogenous rat RFRP-3 does not affect the timing of puberty in male rats but may be associated with peripubertal rise in growth hormone secretion. PMID: 18628614
  10. RFRP-3 is not a hypophysiotropic neuroendocrine hormone in rats. PMID: 19008316

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Subcellular Location
Secreted.
Protein Families
FARP (FMRFamide related peptide) family
Tissue Specificity
Isoform 1 is expressed at high levels in the hypothalamus and eye. Isoform 2 is specifically expressed in a region between the dorsomedial hypothalamic and ventromedial hypothalamic nuclei.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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