Recombinant Rat Fascin (Fscn1)

Code CSB-YP009010RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP009010RA
MSDS
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Source E.coli
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Code CSB-EP009010RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009010RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP009010RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Fscn1
Uniprot No.
Alternative Names
Fscn1Fascin
Species
Rattus norvegicus (Rat)
Expression Region
2-493
Target Protein Sequence
TANGTAEAV QIQFGLISCG NKYLTAEAFG FKVNASASSL KKKQIWTLEQ PPDEAGSAAV CLRSHLGPYL AADKDGNVTC EREVPDGDCR FLVVAHDDGR WSLQSEAHRR YFGGTEDRLS CFAQSVSPAE KWSVHIAMHP QVNIYSVTRK RYAHLSARPA DEIAVDRDVP WGVDSLITLA FQDQRYSVQT SDHRFLRHDG RLVARPEPAT GFTLEFRSGK VAFRDCEGRY LAPSGPSGTL KAGKATKVGK DELFALEQSC AQVVLQAANE RNVSTRQGMD LSANQDEETD QETFQLEIDR DTRKCAFRTH TGKYWTLTAT GGVQSTASTK NASCYFDIEW CERRITLRAS NGKFVTAKKN GQLAATVETA GDSELFLMKL INRPIIVFRG EHGFIGCRKV TGTLDANRSS YDVFQLEFND GAYNIKDSTG KYWTVGSDSS VTSSSDTPVD FFLEFCDYNK VALKVGGRYL KGDHAGVLKA CAETIDPATL WEY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF.
Gene References into Functions
  1. that Fascin-1 increased rapidly in dorsal root ganglions and spinal cord after chronic constriction injury PMID: 29052088
  2. Filopodia formation by crosslinking of F-actin with fascin in two different binding manners PMID: 27169557
  3. There may be a pathological role of fascin in experimental autoimmune neuritis. PMID: 24369046
  4. A tightly regulated phospho/dephospho-fascin cycle modulated by extracellular signals is required for the polarized morphology and migration in neuroblasts, thus contributing to efficient neurogenesis. PMID: 23884926
  5. the expression of fascin-1 is correlated with the migration activity of hepatoblasts during the early stages of liver development in rats. PMID: 18855019

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Subcellular Location
Cytoplasm, cytosol. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell projection, growth cone. Cell projection, filopodium. Cell projection, invadopodium. Cell projection, microvillus. Cell junction.
Protein Families
Fascin family
Tissue Specificity
Detected in embryonic brain and brain from young rats (at protein level).
Database Links

KEGG: rno:683788

UniGene: Rn.199526

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