Recombinant Rat Glutaredoxin-1 (Glrx)

Code CSB-YP875364RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP875364RA
MSDS
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Source E.coli
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Code CSB-EP875364RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP875364RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP875364RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Glrx
Uniprot No.
Alternative Names
Glrx; Glrx1; GrxGlutaredoxin-1; Thioltransferase-1; TTase-1
Species
Rattus norvegicus (Rat)
Expression Region
2-107
Target Protein Sequence
AQEFVNCKI QSGKVVVFIK PTCPYCRKTQ EILSQLPFKR GLLEFVDITA TNNTNAIQDY LQQLTGARTV PRVFIGKDCI GGCSDLLSMQ QNGELTARLK QIGALQL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
Gene References into Functions
  1. Grx1 decreases by ~40% in 2-yr-old Fischer 344 rat hearts vs 6-mo-old controls. Grx1 knockdown in H9c2 cardiomyocytes increases apoptosis, & decreases NFkappaB transcription,& production of Bcl-2 & Bcl-xL. PMID: 19938943
  2. study reports Cys23 of thioltransferase is an essential residue for catalytic activity & the other Cys26 is also important but Cys79 & Cys83 were not essential; for protection of cells from oxidative stress active thioltransferase is essential PMID: 20175144
  3. The presence of enzyme activity in subcellular organelles in brain and liver and in mitochondria demonstrates functional glutaredoxin activity. PMID: 12217626
  4. Glutaredoxin exerts an antiapoptotic effect by regulating the redox state of Akt PMID: 14522978
  5. role of Grx in the maintenance of functional oocytes and in luteal cells surviving the regression process PMID: 15956334
  6. NADPH/glutaredoxin/thioredoxin redox regulation mediates a novel signaling pathway of nutrient-induced insulin secretion. PMID: 15983215
  7. NO increased the S-glutathiolation of SERCA, and adenoviral overexpression of glutaredoxin-1 prevented both the HNO-stimulated oxidative modification of SERCA and its activation. PMID: 19265039
  8. The role of GRX-1 in NADPH-stimulated beta-cell exocytosis and glucose-stimulated insulin secretion, is reported. PMID: 19299446

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Subcellular Location
Cytoplasm.
Protein Families
Glutaredoxin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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