Recombinant Rat Glutathione S-transferase P(Gstp1)

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Code CSB-EP009989RA
Size US$2466Purchase it in Cusabio online store
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Gstp1
Uniprot No. P04906
Research Area Others
Alternative Names Gstp1; Glutathione S-transferase P; EC 2.5.1.18; Chain 7; GST 7-7; GST class-pi
Species Rattus norvegicus (Rat)
Source E.coli
Expression Region 2-210aa
Target Protein Sequence PPYTIVYFPVRGRCEATRMLLADQGQSWKEEVVTIDVWLQGSLKSTCLYGQLPKFEDGDLTLYQSNAILRHLGRSLGLYGKDQKEAALVDMVNDGVEDLRCKYGTLIYTNYENGKDDYVKALPGHLKPFETLLSQNQGGKAFIVGNQISFADYNLLDLLLVHQVLAPGCLDNFPLLSAYVARLSARPKIKAFLSSPDHLNRPINGNGKQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 27.3kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration (By similarity).
Gene References into Functions
  1. The cytotoxic action of 2-acetylaminofluorene that give rise to the GST-P(+) single hepatocytes was suggested to be an injury to the excretory pump(s) and the duct of hepatocytes. A new physiological mechanism was hypothesized for the induction of preneoplastic cell populations in the rat liver instead of a genetic mechanism. PMID: 29501922
  2. Data suggest that expression of Gstp1 can be regulated by dietary factors; here, Gstp1 is induced in hepatocytes by carnosic acid, an anticarcinogenic component of rosemary (Rosmarinus officinalis). PMID: 25974399
  3. Data show that Valerian extract inhibits hepatocarcinogenesis by suppressing cell proliferation and inducing apoptosis in glutathione S-transferase pi GST-P(+) foci by activating gamma-aminobutyric acid A receptor GABA(A)RA1-mediated signaling. PMID: 25419570
  4. Cisplatin metabolism in different organs of rats correlated positively with specific GSTP1 activities and this enzyme may be a critical determinant of extent of cellular uptake or retention of cisplatin in renal and liver tissues. PMID: 24474500
  5. GSTP1 application early after myocardial infarction results in long-term beneficial structural and functional effects that prevent progression to heart faiilure. PMID: 24412522
  6. GST-P(-) foci induced by promotion with agonists of peroxisome proliferator-activated receptor-alpha. PMID: 20423715
  7. GSTpi directly associated with signal transducer and activator of transcription 3 (STAT3) to prevent Angiotensin II-triggered binding of Src to STAT3. PMID: 24321768
  8. The change in expression of GST-pi in peripheral blood showed the same pattern as that in brain tissues, suggesting GST-pi might contribute to drug resistance in epilepsy. PMID: 22038365
  9. GST-P-positive lesions may involve a mechanism facilitating lipid peroxidation PMID: 20091025
  10. Exposure to diesel exhaust particles elevated transcription of glutathione-s-transferase gene in rat alveolar macrophages. PMID: 12011483
  11. GSTpi proteins play an important role in the development of acquired drug resistance to various drugs of prostate cancer cells. PMID: 15809768
  12. glutathione S-transferase P1 is induced by proteasome inhibitors PMID: 15863507
  13. Hepaticcgene expression is upregulated by a limited availability of sulfur amino acids PMID: 15867277
  14. GST-Pi expression was induced by the metabolites of cyclophoshamide. PMID: 16242832
  15. Expression of GST-P gene is down-regulated by CCAAT enhancer-binding protein alpha in normal liver. PMID: 16407263
  16. Drug resistant prostate cancer cells displayed significantly increased expression of GSTP1 with other prostate cancer cells. PMID: 16728343
  17. The upregulation of MIF and GSTpi during the development of acquired drug resistance of hormone independent prostate cancer may modulate the activation of gp-170 PMID: 16728344
  18. Expression of GST-P is correlated with cellular proliferation and is associated with risk and progression of oral cancer. PMID: 17596925
  19. These data indicate that expression of GST-P may reflect the carcinogenic effect of cigarette smoke as well as the genetic susceptibility of animals in relation to continuous carcinogens exposure. PMID: 17786572
  20. development of GST-P-negative foci during the early stage of hepatocarcinogenesis PMID: 18081878

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Subcellular Location Cytoplasm, Mitochondrion, Nucleus
Protein Families GST superfamily, Pi family
Tissue Specificity Present in kidney, lung, testis and placenta, very low levels in liver.
Database Links

KEGG: rno:24426

STRING: 10116.ENSRNOP00000024601

UniGene: Rn.87063

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