Recombinant Rat Kelch repeat and BTB domain-containing protein 10 (Kbtbd10)

Code CSB-YP861687RA
MSDS
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Source Yeast
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Code CSB-EP861687RA
MSDS
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Source E.coli
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Code CSB-EP861687RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861687RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP861687RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Klhl41
Uniprot No.
Alternative Names
Klhl41; Kbtbd10; Krp1; Kelch-like protein 41; Kel-like protein 23; Kelch repeat and BTB domain-containing protein 10; Kelch-related protein 1; Sarcosin
Species
Rattus norvegicus (Rat)
Expression Region
1-606
Target Protein Sequence
MDSQRELAEE LRLYQSTLLQ DGLKDLLEEK KFIDCTLKAG DKSFPCHRLI LSACSPYFRE YFLSEIEEEK KKEMALDNVD PAILDLIIKY LYSASIDLND GNVQDIFALS SRFQIPSVFT VCVSYLQKRL APGNCLAILR LGLLLDCPRL AISAREFVSD RFVQICKEED FMQLSPQELI SVISNDSLNV EKEEVVFEAV MKWVRTDKEN RAKNLSEVFD CIRFRLMAEK YFKDHVEKDD IIKSNPEVQK KIKVLKDAFA GKLPEPSKSA EKGGTGEVNG DVGDEDLLPG YLNDIPRHGM FVKDLILLVN DTAAVAYDPM ENECYLTALA EQIPRNHSSI VTQQNQVYVV GGLYVDEENK DQPLQSYFFQ LDNVSSEWVG LPPLPSARCL FGLGEVDDKI YVVAGKDLQT EASLDSVLCY DPVAAKWSEV KNLPIKVYGH NVISHNGMIY CLGGKTDDKK CTNRVFIYNP KKGDWKDLAP MKTPRSMFGV AIHKGKIVIA GGVTEDGLSA SVEAFDLKTN KWEVMTEFPQ ERSSISLVSL AGSLYAIGGF AMIQLESKEF APTEVNDIWK YEDDKKEWAG MLKEIRYASG ASCLATRLNL FKLSKL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in skeletal muscle development and differentiation. Regulates proliferation and differentiation of myoblasts and plays a role in myofibril assembly by promoting lateral fusion of adjacent thin fibrils into mature, wide myofibrils. Required for pseudopod elongation in transformed cells.
Gene References into Functions
  1. Data show that both knockdown and overexpression of Krp1 inhibit myoblast differentiation assessed by expression of myogenin, MEF2C, MHC, and cell fusion. PMID: 21368295
  2. Krp1, the AP-1 up-regulated protein that has a role in pseudopodial elongation in v-Fos-transformed fibroblast cells, forms a novel interaction with the nondifferentially expressed actin binding protein Lasp-1. PMID: 16449658
  3. binding sites necessary for Krp1-Lasp1 interaction in vitro and function in vivo. PMID: 19726686
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, pseudopodium. Cell projection, ruffle. Cytoplasm, myofibril, sarcomere, M line. Sarcoplasmic reticulum membrane. Endoplasmic reticulum membrane. Note=Predominantly cytoplasmic but can colocalize with F-actin at the membrane ruffle-like structures at the tips of transformation-specific pseudopodia.
Tissue Specificity
Primarily expressed in skeletal muscle. Also found in heart and lung.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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